The Acclaim Carbamate column is designed for baseline separation of carbamates (N-methylcarbamate and N-methylcarbamoyloxime pesticides) specified in US EPA Method 531.2. Carbamate pesticides are widely used throughout the world. Drinking water and raw surface water is monitored for the presence of carbamate pesticides and related compounds using an established EPA Method 531.2 that uses HPLC with postcolumn derivatization. LC-MS is the method of choice for the ultimate sensitivity.
Baseline separation of carbamate pesticides specified in US EPA Method 531.2
Use with either LC/postcolumn derivatization/fluorescence or LC/MS detection.
Compatible with both binary (methanol/water) and ternary (acetonitrile/methanol/water) mobile phase gradients.
High efficiency, extremely low column bleed, and rugged column packing
Acclaim Carbamate columns are available in a variety of column formats: The 5 μm, 4.6 × 250 mm column sets the standard for the highest resolution with postcolumn reaction (PCR) and fluorescence detection (FLD); the 3 μm, 4.6 × 150 mm column saves time and increases sample throughput; the 3 μm, 3.0 × 150 mm column saves time and reduces solvent consumption; and the 3 μm, 2.1 × 150 mm column is for LC/MS, to achieve the lowest possible detection limits. Two RSLC, 2.2 μm columns are available for high-resolution, high-throughput separations: 2.1 × 100 mm and 2.1 × 150 mm.
| Acclaim Carbamate Column Specifications | |
|---|---|
| Column chemistry | Proprietary alkyl group |
| Operating pressure limit (recommended) | 6000 psi (4000 psi) |
| Operating pH range (recommended) | 2.5–8.0 (3.0–7.0) |
| Operating flow rate range (recommended) | 0.5–3.0 (0.8–1.6) mL/min for 4.6 mm i.d. formats 0.3–1.5 (0.4–0.8) mL/min for 3.0 mm i.d. formats 0.15–0.80 (0.2–0.4) mL/min for 2.1 mm i.d. formats |
| Analytical Columns | |
|---|---|
| Acclaim RSLC Carbamate, 2.2μm Analytical (2.1 x 150mm) | 075596 |
| Acclaim RSLC Carbamate, 2.2μm Analytical (2.1 x 100mm) | 075597 |
| Acclaim Carbamate, 5μm Analytical (4.6 x 250mm) | 072924 |
| Acclaim Carbamate, 3μm Analytical (4.6 x 150mm) | 072925 |
| Acclaim Carbamate, 3μm Analytical (3.0 x 150mm) | 072926 |
| Acclaim Carbamate, 3μm Analytical (2.1 x 150mm) | 072927 |
| Guard Columns | |
|---|---|
| Acclaim Carbamate, 3μm Guard (4.6 x 10mm) 2ea (Use Holder V-2) | 072928 |
| Acclaim Carbamate, 3μm Guard (3.0 x 10mm) 2ea (Use Holder V-2) | 072929 |
| Acclaim Carbamate, 3μm Guard (2.1 x 10mm) 2ea (Use Holder V-2) | 072930 |
| Acclaim SST Guard Cartridge Holder V-2 | 069580 |
| Acclaim Guard Kit (Holder and coupler) V-2 | 069707 |
| Guard to Analytical Column Coupler V-2 | 074188 |
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