Acclaim® Rapid Separation Liquid Chromatography (RSLC) columns use 2.2 and 3 μm particles for fast separations. These columns, together with the UltiMate® RSLC system, provide ultrafast, high-efficiency LC separations.
Accelerate separations up to 15-fold compared to conventional LC
Accelerate sample throughput up to 30-fold compared to conventional LC, with Dionex RSLC Solutions
Save up to 85% of solvent with 3 μm columns and 92% of solvent with 2.2 μm columns
Use as little as 15–40% of sample volume traditionally required for LC
Achieve easy-to-use, reliable, fast separations
Ease of operation ensures optimal, fast separations
Acclaim RSLC 2.2 μm columns achieve high throughput without sacrificing chromatographic performance. These columns are based on high-purity spherical porous silica gel. Currently, four column chemistries are available: Acclaim RSLC 2.2 μm C18, C8, PolarAdvantge I (PA) and PolarAdvantage II (PA2).
Acclaim RSLC 2.2 μm C18 material features high-density monomeric C18 chemistry with exhaustive endcapping. As a result, it provides the selectivity of a standard C18 phase and extremely low silanol activity. Similarly, the Acclaim RSLC 2.2 μm C8 material features high-density monomeric C8 chemistry with exhaustive endcapping. The PA material features a polar sulfonamide chemistry that provides very good hydrolytic stability and eliminates dewetting problems. The PA2 material features amide-embedded chemistry with excellent hydrolytic stability (pH 1.5 to 10). Acclaim RSLC 2.2 μm columns are available in 2.1 mm and 3.0 mm i.d. with 30 mm, 50 mm, 100 mm, 150 mm, and 250 mm lengths.
Acclaim RSLC 3 μm columns are available in three stationary phases: Acclaim 120 C18, Polar Advantage (PA), and Polar Advantage II (PA2). The columns are packed in 3 mm i.d. with 33 mm, 50 mm, 75 mm, 150 mm, and 250 mm lengths. The inner diameter of 3 mm makes these columns fully compatible with standard analytical LC instrumentation, and saves up to 85% solvent compared to a 4.6 mm column of otherwise identical features.
Acclaim RSLC columns complement the Dionex UltiMate 3000 RSLC system to provide superior speed, reliability, and ease-of-use. Using RSLC with Dionex LC system solutions for tandem LC, parallel LC, or other techniques further increases sample throughput up to 30-fold. See the LC Solutions sections for more information.
| Acclaim RSLC 2.2 μm Column Specifications | ||
|---|---|---|
| Column | Acclaim® 120 C18 | Acclaim PA2 |
| Substrate | Ultrapure Silica | Ultrapure Silica |
| Organic Solvents | 20-100% | 0-100% |
| Bonded Phase | C18 | Amide C18 |
| Endcapped | Yes | Yes |
| Partical Shape | Spherical | Spherical |
| Particle Size | 2.2μm | 2.2μm |
| Metal Impurity (ppm) NA, Fe, Al | <10 ppm | <10 ppm |
| Average Pore Diameter | 120 ? | 120 ? |
| Surface Area (m2/g) | 320 | 320 |
| Total Carbon Content | 17% | 17% |
| pH Range | 2.0-8.0 | 1.5-10 |
| Pressure Limit (bar) | 600: 2.1 × 50 mm 800: 2.1 × 100mm | 600: 2.1 × 50 mm 800: 2.1 × 100mm |
| Temperature | 80 ° C | 80 ° C |
| Acclaim RSLC 120 C18 Analytical Columns | |
|---|---|
| Acclaim RSLC 120, C18, 3 μm Analytical (3.0 x 33 mm) | 066272 |
| Acclaim 120 C18, 3μm Analytical, (3.0 x 50mm) | 068971 |
| Acclaim RSLC 120, C18, 3 μm Analytical (3.0 x 75 mm) | 066273 |
| Acclaim 120, C18, 3 μm Analyitcal (3.0 x 150 mm) | 063691 |
| Acclaim RSLC 120, C18, 2.2μm Analytical (2.1 x 30 mm) | 071400 |
| Acclaim RSLC 120, C18, 2.2μm Analytical (2.1 x 50 mm) | 068981 |
| Acclaim RSLC 120, C18, 2.2μm Analytical (2.1 x 100 mm) | 068982 |
| Acclaim RSLC 120, C18, 2.2μm Analytical, (2.1 x 150mm) | 071399 |
| Acclaim RSLC 120, C18, 2.2μm Analytical (2.1 x 250mm) | 074812 |
| Acclaim RSLC 120, C18, 2.2 μm Analytical, (3.0 x 30mm) | 071606 |
| Acclaim RSLC 120, C18, 2.2 μm Analytical, (3.0 x 50mm) | 071605 |
| Acclaim RSLC 120, C18, 2.2 μm Analytical, (3.0 x 100mm) | 071604 |
| Acclaim RSLC 120 C8 Analytical Columns | |
|---|---|
| Acclaim RSLC C8, 2.2 μm Analytical (2.1 x 30 mm) | 072614 |
| Acclaim RSLC 120, C8, 2.2μm, 2.1 x 50 mm | 072615 |
| Acclaim RSLC 120, C8, 2.2μm, 2.1 x 100 mm | 072616 |
| Acclaim RSLC 120, C8, 2.2μm, 2.1 x 150 mm | 072617 |
| Acclaim RSLC 120, C8, 2.2μm Analytical (2.1 x 250mm) | 074811 |
| Acclaim RSLC 120, C8, 2.2μm, 3 x 30mm | 072618 |
| Acclaim RSLC 120, C8, 2.2μm, 3 x 50mm | 072619 |
| Acclaim RSLC 120, C8, 2.2μm, 3 x 100mm | 072620 |
| Acclaim RSLC PA Analytical Columns | |
|---|---|
| Acclaim RSLC PA, 3 μm Analytical (3.0 x 33 mm) | 066274 |
| Acclaim PA, 3μm Analytical, (3.0 x 50mm) | 068972 |
| Acclaim RSLC PA, 3 μm Analytical (3.0 x 75 mm) | 066275 |
| Acclaim PA, 3 μm Analyitcal (3.0 x 150 mm) | 063693 |
| Acclaim RSLC PA, 2.2μm, 2.1 x 50 mm | 072622 |
| Acclaim RSLC PA, 2.2μm, 2.1 x 30 mm | 072621 |
| Acclaim RSLC PA, 2.2μm, 2.1 x 100 mm | 072623 |
| Acclaim RSLC PA, 2.2μm, 2.1 x 150 mm | 072624 |
| Acclaim RSLC PA, 2.2μm Analytical (2.1 x 250mm) | 074813 |
| Acclaim RSLC PA, 2.2μm, 3 x 30mm | 072625 |
| Acclaim RSLC PA, 2.2μm, 3 x 100mm | 072627 |
| Acclaim RSLC PA, 2.2μm, 3 x 50mm | 072626 |
| Acclaim RSLC PA2 Analytical Columns | |
|---|---|
| Acclaim RSLC PA2, 3 μm Analytical (3.0 x 33 mm) | 066276 |
| Acclaim PA2, 3μm Analytical, (3.0 x 50mm) | 068973 |
| Acclaim RSLC PA2, 3 μm Analytical (3.0 x 75 mm) | 066277 |
| Acclaim PA2, 3 μm Analyitcal (3.0 x 150 mm) | 063705 |
| Acclaim RSLC PA2, 2.2μm Analytical, (2.1 x 30mm) | 071402 |
| Acclaim RSLC PA2, 2.2μm Analytical (2.1 x 50 mm) | 068989 |
| Acclaim RSLC PA2, 2.2μm Analytical (2.1 x 100 mm) | 068990 |
| Acclaim RSLC PA2, 2.2μm Analytical, (2.1 x 150mm) | 071401 |
| Acclaim RSLC PA2, 2.2μm Analytical (2.1 x 250mm) | 074814 |
| Acclaim RSLC PA2, 2.2 μm Analytical, (3.0 x 100mm) | 071607 |
| Acclaim RSLC PA2, 2.2 μm Analytical, (3.0 x 50mm) | 071608 |
| Acclaim RSLC PA2, 2.2 μm Analytical, (3.0 x 30mm) | 071609 |
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