新!XBridge Protein BEH SEC HPLC色谱柱
蛋白质SEC表征的方法转换
2010年,沃特世推出了1.7 μm的ACQUITY UPLC Protein BEH SEC色谱柱,用于在沃特世低扩散的UPLC仪器上获得最佳性能。与传统的5-8 μmSEC色谱柱相比,ACQUITY UPLC Protein BEH SEC色谱柱首次在更短时间内表现出了卓越的组分分离度。2015年,沃特世推出化学性质完全一致的XBridge Protein BEH SEC 125?、200?和450?, 3.5 μm色谱柱,可在常规HPLC平台上提供相当的组分分离度。
沃特世XBridge? Protein BEH SEC 125?、200?和450?, 3.5 μm色谱柱设计用于HPLC仪器,互补现有的用于蛋白分离的小粒径UPLC?体积排阻色谱柱(SEC)系列。这类新型HPLC SEC色谱柱基于相同的沃特世亚乙基桥杂化颗粒(BEH)和稳定的二醇基键合技术,与传统100%硅胶基质SEC产品相比性能更佳。因此,色谱工作者现在能够根据实验室仪器、所需蛋白组分分离度和样品通量的要求,轻松地开发和/或转换SEC方法。
? 用于SEC HPLC分离1K-1,500 kDa范围内的肽、蛋白质,具有更高的通量
? 卓越的SEC色谱柱寿命
? 与硅胶基质SEC色谱柱相比,显著减少不希望发生的蛋白与色谱柱之间的相互作用
? 全面的测试可保证无可比拟的柱间一致性,并提高了已验证方法的可靠性
? 互补ACQUITY UPLC? SEC色谱柱系列,可根据不同的应用需求实现方法的无缝转换
蛋白反相分离技术专用柱BEH 300 C4 UPLC和HPLC柱
蛋白质样品的分析和鉴定需要检出大分子蛋白质之间的微小化学差异。通常,这类分析使用一系列分析技术,每种分析技术对蛋白质的不同属性敏感。反相HPLC并未完全被纳入这些测试手段,因为对蛋白质的反相分离通常会产生相对较宽和不对称的峰形,而且蛋白质的回收率低、残留问题显著。沃特世的新型反相、亚乙基桥杂化颗粒(BEH技术)的蛋白质分离技术色谱柱,专设计用于对蛋白质进行高分辨率分析。
沃特世BEH300 C4柱用于蛋白质分离:
??分离大小不同、疏水性和等电点各异的蛋白质
??可提供用于HPLC方法的3.5 μm填料和用于UPLC方法的1.7 μm填料
??使回收最大化,蛋白质残留最小
??可耐受极端pH值和温度
??使用蛋白混标进行质控
??可直接接入ESI-MS用于蛋白确认
ACQUITY UPLC Protein BEH300 C4与XBridge BEH300 C4 分析柱
产品描述 | 粒径 | 柱规格 | 部件编号 |
ACQUITY UPLC Protein BEH300 C4 | 1.7 μm | 1.0 x 50 mm | 186005589 |
ACQUITY UPLC Protein BEH300 C4 | 1.7 μm | 1.0 x 100 mm | 186005590 |
ACQUITY UPLC Protein BEH300 C4 | 1.7 μm | 1.0 x 150 mm | 186005591 |
ACQUITY UPLC Protein BEH300 C4 | 1.7 μm | 2.1 x 50 mm | 186004495 |
ACQUITY UPLC Protein BEH300 C4 | 1.7 μm | 2.1 x 100 mm | 186004496 |
ACQUITY UPLC Protein BEH300 C4 | 1.7 μm | 2.1 x 150 mm | 186004497 |
ACQUITY UPLC Protein BEH300 C4 | 1.7 μm | 预柱 | 186004623 |
ACQUITY UPLC Protein BEH300 C4方法验证包* | 1.7 μm | 2.1 x 100 mm | 186004899 |
ACQUITY UPLC Protein BEH300 C4方法验证包 | 1.7 μm | 2.1 x 150 mm | 186006549 |
nanoACQUITY Protein BEH300 C4 | 1.7 μm | 75 μm x 100mm | 186004639 |
nanoACQUITY Protein BEH300 C4 | 1.7 μm | 100 μm x 100mm | 186004640 |
nanoACQUITY Protein BEH300 C4 | 1.7 μm | 150 μm x 100mm | 186004641 |
XBridge Protein BEH300 C4 | 3.5 μm | 2.1 x 50 mm | 186004498 |
XBridge Protein BEH300 C4 | 3.5 μm | 2.1 x 100 mm | 186004499 |
XBridge Protein BEH300 C4 | 3.5 μm | 2.1 x 150 mm | 186004500 |
XBridge BEH300 C4 | 3.5 μm | 2.1 x 250 mm | 186004501 |
XBridge Protein BEH300 C4 | 3.5 μm | 4.6 x 50 mm | 186004502 |
XBridge Protein BEH300 C4 | 3.5 μm | 4.6 x 100 mm | 186004503 |
XBridge Protein BEH300 C4 | 3.5 μm | 4.6 x 150 mm | 186004504 |
XBridge Protein BEH300 C4 | 3.5 μm | 4.6 x 250 mm | 186004505 |
XBridge Protein BEH C4 Sentry保护柱 | 3.5 μm | 2.1 x 10mm | 1860072301 |
XBridge Protein BEH C4 Sentry保护柱 | 3.5 μm | 4.6 x 20mm | 1860072352 |
XBridge Protein BEH300 C4方法验证包 | 3.5 μm | 4.6 x 100 mm | 186005465 |
*包含三根色谱柱,由不同批次填料装填
1要求2.1 x 10mm通用Sentry保护柱套,部件编号:WAT097958
2要求4.6 x 20mm 通用Sentry保护柱套,部件编号:WAT046910
注意:ACQUITY UPLC BEH300 C4 1.7μm色谱柱设计用于ACQUITY UPLC系统。其亚二微米小颗粒的
色谱柱优势只有在低系统体积和低检测器谱带扩散的ACQUITY UPLC系统上才能实现。
XBridge Protein BEH300 C4制备柱
产品描述 | 部件编号 |
XBridge Protein BEH300 C4 5 μm 10 x 10 mm Guard Cartrigge | 186007305 |
XBridge Protein BEH300 C4 5 μm 10 x 50 mm Column | 186007306 |
XBridge Protein BEH300 C4 5 μm 10 x 100 mm Column | 186007307 |
XBridge Protein BEH300 C4 5 μm 10 x 150 mm Column | 186007308 |
XBridge Protein BEH300 C4 5 μm 10 x 250 mm Column | 186007309 |
XBridge Protein BEH300 C4 5 μm 19 x 10 mm Guard Cartrigge | 186007310 |
XBridge Protein BEH300 C4 5 μm OBD 19 x 50 mm Column | 186007311 |
XBridge Protein BEH300 C4 5 μm OBD 19 x 100 mm Column | 186007312 |
XBridge Protein BEH300 C4 5 μm OBD 19 x 150 mm Column | 186007313 |
XBridge Protein BEH300 C 4 5 μm OBD 19 x 250 mm Column | 186007314 |
XBridge Protein BEH300 C4 5 μm 30 x 10 mm Guard Cartrigge | 186007315 |
XBridge Protein BEH300 C4 5 μm OBD 30 x 50 mm Column | 186007316 |
XBridge Protein BEH300 C4 5 μm OBD 30 x 75 mm Column | 186007317 |
XBridge Protein BEH300 C4 5 μm OBD 30 x 100 mm Column | 186007318 |
XBridge Protein BEH300 C4 5 μm OBD 30 x 150 mm Column | 186007319 |
XBridge Protein BEH300 C4 5 μm OBD 30 x 250 mm Column | 186007320 |
XBridge Protein BEH300 C4 10 μm 10 x 10 mm Guard Cartrigge | 186007325 |
XBridge BEH300 C4 10 μm 10 x 50 mm Column | 186007326 |
XBridge BEH300 C4 10 μm 10 x 100 mm Column | 186007327 |
XBridge BEH300 C4 10 μm 10 x 150 mm Column | 186007328 |
XBridge Protein BEH300 C4 10 μm 10 x 250 mm Column | 186007329 |
XBridge Protein BEH300 C4 10 μm 19 x 10 mm Guard Cartrigge | 186007330 |
XBridge Protein BEH300 C4 10 μm OBD 19 x 50 mm Column | 186007331 |
XBridge Protein BEH300 C4 10 μm OBD 19 x 100 mm Column | 186007332 |
XBridge Protein BEH300 C4 10 μm OBD 19 x 150 mm Column | 186007333 |
XBridge Protein BEH300 C4 10 μm OBD 19 x 250 mm Column | 186007334 |
XBridge Protein BEH300 C4 10 μm 30 x 10 mm Guard Cartrigge | 186007335 |
XBridge Protein BEH300 C4 10 μm OBD 30 x 50 mm Column | 186007336 |
XBridge Protein BEH300 C4 10 μm OBD 30 x 75 mm Column | 186007337 |
XBridge Protein BEH300 C4 10 μm OBD 30 x 100 mm Column | 186007338 |
XBridge Protein BEH300 C4 10 μm OBD 30 x 150 mm Column | 186007339 |
XBridge Protein BEH300 C4 10 μm OBD 30 x 250 mm Column | 186007340 |