GlycanPac AXR-1 多糖分析柱
Thermo Scientific GlycanPac AXR-1列是由电荷，异构和大小聚糖的同时分离一个高性能，二氧化硅基HPLC柱。它是专为生物重要聚糖提供独特的选择性业界领先的分辨率，无论是标或本机，或者使用荧光和/或质谱（MS）检测。
GlycanPac AXR-1 多糖分析柱
订货信息：
| 分析柱 | |
| GlycanPac AXR-1, Analytical, 1.9μm, 2.1x150mm | 088136 |
| GlycanPac AXR-1, Analytical, 1.9μm, 2.1x250mm | 088135 |
| GlycanPac AXR-1, Analytical, 3.0μm, 2.1x150mm | 088251 |
| GlycanPac AXR-1, Analytical, 3.0μm, 3.0x150mm | 088252 |
| GlycanPac AXR-1, Analytical, 3.0μm, 4.6 x150mm | 088255 |
| 保护柱 | |
| GlycanPac AXR-1, Guard, 3.0μm, 2.1 x10mm,2EA | 088258 |
| GlycanPac AXR-1, Guard, 3.0μm, 3.0 x10mm,2EA | 088259 |
| GlycanPac AXR-1, Guard, 3.0μm, 4.6 x10mm,2EA | 088260 |
| Acclaim SST Guard Cartridge Holder V-2 | 069580 |
| Acclaim Guard Kit (Holder and coupler) V-2 | 069707 |
| Guard to Analytical Column Coupler V-2 | 074188 |
GlycanPac AXR-1 Columns for the High-Resolution Separation and Analysis of Glycans
The Thermo Scientific™ GlycanPac™ AXR-1 column is a high-performance, silica-based HPLC column for the simultaneous separation of glycans by charge, isomerism, and size. It is designed to provide industry-leading resolution with unique selectivities for biologically important glycans, either labeled or native, using either fluorescence and/or mass spectrometry (MS) detection.
Features:
? Unique glycan selectivity based on charge, branch/linkage isomerism, and
size
? Exceptional resolution for either native or derivatized glycans
?
Utility for both high-resolution glycan structural characterization and glycan
quantification
? Compatibility with both MS and fluorescence detection
methods
? Reliable column performance
? Available in HPLC (3 μm) and UHPLC
(1.9 μm) formats.
The GlycanPac AXR-1 column, based on innovative, mixed-mode surface chemistry, combines both weak anion-exchange (WAX) and reversed-phase interaction mechanisms to produce exceptional glycan selectivity and industry-leading resolution. The WAX functionality provides retention of negatively charged glycans that elute in order of increasing charge, and the reversed-phase mode facilitates resolution of glycans of the same charge according to their isomerism and size. These attributes produce unparalleled glycan separations. The GlycanPac AXR-1 column is designed for HPLC and UHPLC methods using either fluorescence or MS detection, and uses volatile aqueous buffers (e.g., ammonium acetate or ammonium formate) and acetonitrile, presenting the eluting glycans ready for introduction into MS instruments.
GlycanPac AXR-1 columns are based on high-purity and spherical silica substrates in both 1.9 and 3 μm sizes for UHPLC and standard HPLC applications, respectively. GlycanPac AXR-1 columns are available in various dimensions for applications with different resolutions, throughput, and instrument requirements.
| Analytical Columns | |
|---|---|
| GlycanPac AXR-1, Analytical, 1.9μm, 2.1x150mm | 088136 |
| GlycanPac AXR-1, Analytical, 1.9μm, 2.1x250mm | 088135 |
| GlycanPac AXR-1, Analytical, 3.0μm, 2.1x150mm | 088251 |
| GlycanPac AXR-1, Analytical, 3.0μm, 3.0x150mm | 088252 |
| GlycanPac AXR-1, Analytical, 3.0μm, 4.6 x150mm | 088255 |
| Guard Columns | |
|---|---|
| GlycanPac AXR-1, Guard, 3.0μm, 2.1 x10mm,2EA | 088258 |
| GlycanPac AXR-1, Guard, 3.0μm, 3.0 x10mm,2EA | 088259 |
| GlycanPac AXR-1, Guard, 3.0μm, 4.6 x10mm,2EA | 088260 |
| Acclaim SST Guard Cartridge Holder V-2 | 069580 |
| Acclaim Guard Kit (Holder and coupler) V-2 | 069707 |
| Guard to Analytical Column Coupler V-2 | 074188 |
| DataSheet | |
|---|---|
 | GlycanPac AXR-1 Columns For High-Resolution Glycan Analysis |
| Brochures | |
|---|---|
| Thermo Scientific BioLC Columns |
| Manuals | |
|---|---|
| Thermo Scientific GlycanPac AXR-1 Columns Product Manual |
| GLYCANPAC AXR-1 COLUMN Quick Start |
| Posters | |
|---|---|
| An Ultra High Resolution Glycan Column for Isomeric Separation and the Structural Identification of Labeled N-Glycans from Proteins Including Antibodies |
| GlycanPac AXR-1 Column Specifications | |
|---|---|
| Column Dimensions | 1.9 μm: 2.1 × 250mm, 2.1 × 150mm 3 μm: 4.6 × 150mm, 3.0 × 150mm, 2.1 × 150mm |
| Particle Size | Spherical 1.9 μm, 3 μm particles |
| Substrate | High-purity, Porous, Spherical Silica |
| Surface Area | 220 m2/g |
| Pore size | 175 ? |
| Functional Group | WAX and RP Mixed-Mode |
| Recommended Flow Rate Range: | 1.9 μm: 0.2 to 0.4 mL/min. for: all 2.1 mm columns 3 μm: 0.8 to 1.2 ml/min for 4.6 × 150 mm 0.3 to 0.6 ml/min for 3.0 × 150 mm 0.1 to 0.3 ml/min. for 2.1 × 150 mm |
| Pressure Limit | 1.9 μm: 10,000 psi for 2.1 ×150mm 15,000 psi for 2.1 × 250mm 3 μm: 6,000 psi for all 3 μm columns |
| Temperature Range: | Ambient to < 60 oC |
| pH Range | 2.0 - 8.0 |
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