Dionex CarboPac PA1 糖和氨基酸分析柱
对于各种基质中单糖、双糖和线性多糖的测定，使用耐用的 Thermo Scientific? Dionex? CarboPac? PA1 分析和保护柱。 这款专用的阴离子交换柱与脉冲电流检测技术 (PAD) 结合使用，可提供高分辨率的分离。 Dionex CarboPac PA1 树脂的独特 Thermo Scientific? Dionex? MicroBead? 薄壳结构，使其在所有缓冲盐溶液中可保持 pH 0–14 的稳定性。 薄壳的树脂结构提供出色的质量传递优秀的传质，从而实现快速的梯度再平衡。
用于碳水化合物分离的耐用型柱
经验证适用于各种正式的食物分析方法
特别适合针对营养标签进行的食物分析
非常适用于中性与酸性单糖的分离
灵敏度低于 Dionex CarboPac PA10 或 PA20 柱
提供微孔、标准孔和半制备规格
关于 Dionex CarboPac 柱系列
Dionex CarboPac 系列提供精选的高性能液相色谱 (HPLC) 柱，每一款均为不同类别化合物而优化。 与能够检测碳水化合物而不产生衍生物的 PAD 配合使用，这些色谱柱支持简单可靠的糖醇、单糖和双糖、多糖和低聚糖分离技术。
Dionex CarboPac 柱提供以下成分的高分辨率分离：
膳食纤维中的糖蛋白单糖和复合碳水化合物，包括果聚糖、麦芽糊精和支链淀粉
单糖，例如从糖蛋白水解物和植物水解物得到的单糖
单糖和双糖
用途和特点
AminoPac PA1柱用于氨基酸的快速分离。特别适用于分析酸性和酸不稳定性氨基酸，以及用阳离子交换分离不完全的氨基酸对。
Dionex CarboPac PA1 糖和氨基酸分析柱
订货信息：
| 分析柱 | |
| CarboPac PA1 Analytical Column (2 x 250 mm) | 057178 |
| CarboPac PA1 Analytical Column (4 X 250 mm) | 035391 |
| CarboPac PA1 Semi-Preparative Column (9 x 250 mm) | 039686 |
| CarboPac PA1 Semi-Preparative Column (22 x 250 mm) | 0SP2866 |
| 保护柱 | |
| CarboPac PA1 Guard Column (2 x 50 mm) | 057179 |
| CarboPac PA1 Guard Column (4 x 50 mm) | 043096 |
| CarboPac PA1 Guard (9 x 50 mm) | 063501 |
The CarboPac? PA1 is an anion-exchange column for the separation of mono-, disaccharides, and specific oligosaccharides using an isocratic eluent. Sialic acid analysis has also been demonstrated on this column.
General-purpose column for isocratic determination of monosaccharides and disaccharides
Detection by pulsed amperometric detection; no derivatization needed
Approved for use in a variety of official methods for the analysis of foods
The unique MicroBead? pellicular structure of the CarboPac PA1 resin gives it stability from pH 0–14 at all concentrations of buffer salts. The pellicular resin structure allows excellent mass transfer resulting in fast gradient reequilibration.
The CarboPac PA1 column is used with pulsed amperometric detection; no derivatization is required. Sensitivity on the PA1 is not as high as with the CarboPac PA20 or PA10 for monosaccharide analysis. Thus the CarboPac PA1 is well suited for the analysis of foods for nutritional labeling. The CarboPac PA1 has been approved for use in a number of official methods.
| CarboPac PA1 Column for Routine Carbohydrate Analysis Specifications | |
|---|---|
| Dimensions | CarboPac PA1 Analytical Column: 4 × 250 mm and 2 × 250 mm CarboPac PA1 Guard Column: 4 × 50 mm and 2 × 50 mm CarboPac PA1 Semipreparative Column: 22 × 250 mm |
| Maximum operating pressure | 4000 psi (27.9 MPa) |
| Temperature range | 4–55 °C Recommended operating temperature: ambient |
| Typical operating conditions | 1500 psi at 1.0 mL/min (analytical and guard columns) Recommended flow rate: 1.0 mL/min (for 4 × 250 mm) |
| Mobile phase compatibility | pH 0–14; up to 2% of common HPLC solvents. Acetate or hydroxide eluents only. |
| Resin composition | 10-μm-diameter substrate (polystyrene 2% cross-linked with divinylbenzene) agglomerated with 500-nm MicroBead quaternary ammonium functionalized latex (5% cross-linked). |
| Anion-exchange capacity | Approximately 100 μeq/column (4 × 250 mm analytical column) |
| Column construction | PEEK with 10-32 threaded-ferrule-style end fittings. All components are nonmetallic. |
Chromatographic analysis of purified inulin.
Analysis of mono- and diphosphorylated monosaccharides on the CarboPac PA1.
Isocratic Separation of Sugars Found in Sugarcane Molasses Using the CarboPac PA1 Column
| Manuals | |
|---|---|
 | Dionex CarboPac PA1 Column Product Manual |
| Product Data Sheets | |
|---|---|
| Dionex CarboPac PA1 Column for Mono-, Di- and Oligosaccharide Analysis |
| Application Updates | |
|---|---|
| AU 150: Determination of Plant-Derived Neutral Oligo- and Polysaccharides Using the CarboPac PA200 |
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