The CarboPac® MA1 column is a high-capacity, strong anion-exchange column that delivers sensitive, rugged, and reliable separations of reduced sugars without the need for derivatization. The MA1 consists of a macroporous resin fully functionalized with a tertiary amine.
Recommended for the separation of reduced mono- and disaccharides
No derivatization needed
Separations using a simple hydroxide eluent
Alditols and aldoses separated from one another in a single run
Designed for reduced mono- and disaccharide alditol analyses, the CarboPac MA1 is also well suited for analysis of exoglycosidase-released neutral monosaccharides. Monosaccharides are moved away from the column void, enabling immediate evaluation of contaminating exoglycosidase activities. The MA1 can provide baseline resolution of fucose, N-acetyl-(D)-glucosamine, N-acetyl-galactosamine, mannose, glucose, and galactose, as well as neutral oligosaccharides in the same separation.
| CarboPac MA1 Column for Carbohydrate Alditol and Aldose Analysis Specifications | |
|---|---|
| Dimensions | CarboPac MA1 Analytical Column: 4 × 250 mm CarboPac MA1 Guard Column: 4 × 50 mm |
| Maximum operating pressure | 2000 psi (14 MPa) |
| Temperature range | 4–50 °C Recommended operating temperature: ambient |
| Typical operating conditions | Recommended flow rate: 0.2–0.5 mL/min |
| Mobile phase compatibility | pH 0–14; incompatible with solvents and anionic detergents. Hydroxide eluents only. |
| Resin composition | 7.5-μm-diameter vinylbenzyl chloride/divinylbenzene macroporous substrate fully functionalized with an alkyl quaternary ammonium group (15% cross-linked). |
| Anion-exchange capacity | Approximately 1450 μeq/column (4 × 250 mm analytical column) |
| Column construction | PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic. |
| Analytical Columns | |
|---|---|
| CarboPac MA1 Analytical Column (4 x 250 mm) | 044066 |
| Guard Columns | |
|---|---|
| CarboPac MA1 Guard Column (4 x 50 mm) | 044067 |
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