Oligosaccharides released from therapeutic glycoproteins can differ in size, charge, branching, and linkage. Oligosaccharides with these features can be resolved using the CarboPac® PA100. It provides the flexibility to separate oligosaccharides into broad classes depending upon the degree of sialylation, while maintaining the resolution needed for routine analysis based on linkage isomerism. Coupled with pulsed amperometric detection, the PA100 supports detection at low-picomole levels.
Separation of closely related oligosaccharides, even isomers, at picomole levels
Separation of neutral and charged oligosaccharides in the same run
No sample derivatization required for detection
The CarboPac PA100 column is a high-resolution, strong anion-exchange column for enhanced chromatography of oligosaccharides.
The Carbohydrate Membrane Desalter can be used on-line prior to fraction collection.
| CarboPac PA100 Column for Oligosaccharide Analysis Specifications | |
|---|---|
| Dimensions | CarboPac PA100 Analytical Column: 4 × 250 mm CarboPac PA100 Guard Column: 4 × 50 mm CarboPac PA100 Microbore Column: 2 × 250 mm CarboPac PA100 Microbore Guard Column: 2 × 50 mm CarboPac PA100 Semipreparative Column: 9 × 250 mm CarboPac PA100 Semipreparative Column: 22 × 250 mm |
| Maximum operating pressure | 4000 psi (27.9 MPa) |
| Temperature range | 4–60 °C Recommended operating temperature: ambient |
| Typical operating conditions | 2400 psi at 1.0 mL/min (analytical and guard columns) Recommended flow rate: 1.0 mL/min |
| Mobile phase compatibility | pH 0–14; 100% compatible with common organic solvents. Ionic form eluents: sodium acetate and sodium hydroxide only. |
| Resin composition | 8.5-μm-diameter ethylvinylbenzene/divinylbenzene substrate (55% cross-linking) agglomerated with 275-nm MicroBead™ quaternary amine functionalized latex (6% cross-linked). |
| Anion-exchange capacity | Approximately 90 μeq/column (4 × 250 mm analytical column) |
| Column construction | PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic. |
| Analytical Columns | |
|---|---|
| CarboPac PA100 Analytical Column (2 x 250 mm) | 057182 |
| CarboPac PA100 Analytical Column (4 x 250 mm) | 043055 |
| CarboPac PA100 Column (9 x 250 mm) | SP2089 |
| CarboPac PA100 Column (22 x 250 mm) | SP2667 |
| Guard Columns | |
|---|---|
| CarboPac PA100 Guard Column (2 x 50 mm) | 057183 |
| CarboPac PA100 Guard Column (4 x 50 mm) | 043054 |
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