Dionex CarboPac PA200 碳水化合物分析柱
对于高分辨率测绘和糖蛋白释放的寡糖的分析，使用的Thermo Scientific戴安CARBOPACPA200分析及保护柱提供的敏感性和特异性检测。脉冲安培检测（PAD）相结合，此阴离子交换柱提供无需使用衍生快速，重复分离。疏水性聚合物，薄壳型阴离子交换树脂填充材料是在pH值0-14的范围内保持稳定。这种独特的pH值稳定性允许使用有利于碳水化合物的阳极氧化的金电极洗脱液组成的。
Dionex CarboPac PA200 碳水化合物分析柱
订货信息：
| 分析柱 | |
| CarboPac PA200 Analytical Column (3 x 250 mm) | 062896 |
| 保护柱 | |
| CarboPac PA200 Guard Column (3 x 50 mm) | 062895 |
A variety of HPLC approaches are proposed for characterizing oligosaccharides, but many prove inadequate for separating complex mixtures and are limited by a lack of specificity and high limits of detection. Dionex has developed an improved HPLC technique for carbohydrate analysis based on anion-exchange chromatography coupled with pulsed amperometric detection. This technique provides high-resolution separations with sensitive and specific detection, without derivatization.
Sensitive, direct detection with no derivatization, using pulsed amperometric detection
Very high-efficiency separations
Separations based on size, charge, degree of branching, and linkage isomerism
Low flow rate saves on eluent consumption
Does not require derivatization
CarboPac? PA200 columns are packed with a hydrophobic, polymeric, pellicular anion-exchange resin stable over the range of pH 0–14. The unique pH-stability of the packing material allows the use of eluent compositions that are conducive to anodic oxidation of carbohydrates at gold electrodes.
| CarboPac PA200 Column for High-Resolution Oligosaccharide Separations Specifications | |
|---|---|
| Dimensions | CarboPac? PA200 Analytical Column: 3 × 250 mm CarboPac PA200 Guard Column: 3 × 50 mm |
| Maximum operating pressure | 4000 psi (27.9 MPa) |
| Temperature range | 6–60 °C Recommended operating temperature: controlled ambient |
| Typical operating conditions | 2700 psi at 0.5 mL/min (analytical and guard columns) Recommended flow rate: 0.5 mL/min |
| Mobile phase compatibility | pH 0–14; 100% compatible with common organic solvents. Ionic form eluents: sodium acetate and sodium hydroxide only. |
| Resin composition | 5.5 μm-diameter ethylvinylbenzene/divinylbenzene substrate (55% cross-linked) agglomerated with 43 nm MicroBead? quaternary amine functionalized latex (6% crosslinked) |
| Anion-exchange capacity | Approximately 35 μeq/column (3 × 250 mm analytical column) |
| Column construction | PEEK? with 10-32 threaded ferrule-style end fittings. All components are nonmetallic |
| Product Data Sheets | |
|---|---|
 | CarboPac PA200 Column Solutions for Oligosaccharide Analysis Data Sheet |
| Application Updates | |
|---|---|
| AU 150: Determination of Plant-Derived Neutral Oligo- and Polysaccharides Using the CarboPac PA200 |
| AU 176: Preparation of Peptide N-Glycosidase F Digests for HPAE-PAD Analysis |
| Manuals | |
|---|---|
| CarboPac PA200 Analytical Column Manual |
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