The OmniPac® PAX-500 column is a macroporous, low-capacity, solvent-compatible column designed to separate anionic and neutral species in a single run. This column combines both anion-exchange and polymeric reversed-phase retention mechanisms in a single column.
Good for the simultaneous separation of neutral and anionic species
Unique selectivity for polar and ionic organic analytes
Effective for biological samples such as purines, pyrimidines, and vitamins
Good for drugs and pharmaceuticals
The multimode separation mechanism of the OmniPac PAX-500 is achieved by coating a macroporous, hydrophobic core for reversed-phase retention, with an anion-exchange latex bead layer for anion-exchange retention. The macroporous structure provides an accessible hydrophobic core where reversed-phase retention occurs. The anion-exchange selectivity is provided by an anion-exchange MicroBead™ latex that coats the hydrophobic core. Since the anion-exchange layer is identical to that of the OmniPac PAX-100, the two columns exhibit similar ion-exchange selectivity. As a result, the OmniPac PAX-100 can be used to develop the ion-exchange separation and the method can then be transferred to the OmniPac PAX-500 for refinement to include the reversed-phase separation. The OmniPac PAX-500 column is 100% solvent compatible and should always be run with at least 1% organic solvent in the mobile phase to maintain column life.
The OmniPac PAX-500 may be used for the separation of compounds such as alcohols, alkanolamines, antihistamines, anti-inflammatory agents, aromatic acids, inorganic acids, peptides, purines and pyrimidines, sulfonamides, sulfonated anionic surfactants, and water-soluble vitamins.
| OmniPac PAX-500 Multimode Column Specifications | |
|---|---|
| Dimensions | OmniPac PAX-500 Analytical Column: 4 × 250 mm OmniPac PAX-500 Guard Column: 4 × 50 mm |
| Maximum operating pressure | 4000 psi (27.9 MPa) |
| Recommended operating temperature | Ambient |
| Typical operating conditions | 1.0 mL/min with at least 1% organic solvent Recommended flow rate: 1.0 mL/min |
| Mobile phase compatibility | 100% compatible with common organic solvents. Recommended eluents: solvent for reversed-phase, anion, such as Cl– or OH– for anion-exchange. |
| Resin composition | 8.5-μm-diameter ethylvinylbenzene/divinylbenzene macroporous substrate (55% cross-linked) agglomerated with 60-nm MicroBead alkanolquaternary amine-functionalized latex (4% cross-linked). |
| Anion-exchange capacity | Approximately 40 μeq/column (4 × 250 mm analytical column) |
| Column construction | PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic. |
| Analytical Columns | |
|---|---|
| OmniPac PAX-500 Analytical Column (4 x 250 mm) | 042152 |
| Guard Columns | |
|---|---|
| OmniPac PAX-500 Guard Column (4 x 50 mm) | 042153 |
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