The OmniPac® PCX-500 column is a macroporous, high-capacity, solvent-compatible column designed to separate cationic and neutral species in a single run. This column combines cation-exchange and polymeric reversed-phase retention mechanisms in a single column.
Good for the simultaneous separation of neutral and cationic species
Acid and base compatible, pH 0–14
100% solvent compatible
Not as effective as the CS12A for inorganic cations
The multimode separation mechanism is achieved by coating a macroporous hydrophobic core for reversed-phase retention with a cation-exchange latex bead layer for cation-exchange retention. The macroporous structure provides an accessible hydrophobic core where reversed-phase retention occurs. The cation-exchange selectivity is provided by a cation-exchange MicroBead™ layer that coats the hydrophobic core. Since the cation-exchange layer is identical to that of the OmniPac PCX-100, the two columns exhibit similar ion-exchange selectivity. As a result, the OmniPac PXC-100 can be used to develop the ion-exchange separation, and the method can then be transferred to the OmniPac PXC-500 for further refinement to include solvent for the reversed-phase separation. The OmniPac PCX-500 is 100% solvent compatible and should always be run with at least 1% organic solvent in the mobile phase to maintain column life.
The OmniPac PCX-500 may be used for the separation of compounds such as adrenergics, alcohols, anilines, antidepressants, anti-inflammatory agents, plating bath brighteners, cephalosporins, stains and dyes, herbicides, nucleotides and nucleosides, peptides, sulfonamides, and water-soluble vitamins.
| OmniPac PCX-500 Mixed-Mode Column Specifications | |
|---|---|
| Dimensions | OmniPac PCX-500 Analytical Column: 4 × 250 mm OmniPac PCX-500 Guard Column: 4 × 50 mm |
| Maximum operating pressure | 4000 psi (27.9 MPa) |
| Recommended operating temperature | Ambient |
| Typical operating conditions | 1.0 mL/min with at least 1% organic solvent Recommended flow rate: 1.0 mL/min |
| Mobile phase compatibility | 100% compatible with common organic solvents. Recommended eluents: 1% solvent. Acid to maintain weak bases in the cationic form, salt (e.g., Na+ for cation exchange). |
| Resin composition | 8.5-μm-diameter ethylvinylbenzene/divinylbenzene macroporous substrate (55% cross-linked) agglomerated with 200-nm MicroBead sulfonic acid-functionalized latex (5% cross-linked). |
| Anion-exchange capacity | 120 μeq/column (4 × 250 mm analytical column) |
| Column construction | PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic. |
| Analytical Columns | |
|---|---|
| OmniPac PCX-500 Analytical Column (4 x 250 mm) | 042191 |
| Guard Columns | |
|---|---|
| OmniPac PCX-500 Guard Column (4 x 50 mm) | 042195 |
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