平台编号:Bio-132124
Malassezia sympodialis Simmons et Guého 拉丁名
ATCC 96803 编号
Product category Fungi
Product type Yeast
Strain designation 别名 EG 604 [CBS 7222, GSU 8541]
Type strain 模式菌株 Yes
Isolation 分离源 source Healthy ear Geographical isolation United States; Georgia; Atlanta
Product format 提供形式 Frozen
Storage conditions 保藏温度 -80°C or colder
Specific applications Biomedical Research and Development Material
Preceptrol No
Medium 培养基 ATCC Medium 2693: modified Dixon (mDixon)
ATCC Medium 2737: Modified Leeming & Notman Agar (MLNA)
Temperature 培养温度 24-26°C
Atmosphere 需氧情况 Aerobic
Handling procedure Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week.
Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C).
Storage of frozen material at this temperature will result in the death of the culture.
To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes).
Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µL (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended.
Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1-2 days of incubation.
However, the time necessary for significant growth will vary from strain to strain.
Deposited as Malassezia sympodialis Simmons et Gueho Depositors E Gueho
Chain of custody ATCC <-- E Gueho <-- R.B. Simmons GSU 8541
Type of isolate Human Special collection NCRR Contract
Cross references 参考文献 GenBank AF064024 26S ribosomal RNA gene, partial sequence GenBank AJ249953 26S ribosomal RNA gene, partial sequence
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