新型隐球菌Cryptococcus neoformans

平台编号：Bio-133994

Cryptococcus neoformans (Sanfelice) Vuillemin

24067™

An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.

Product category

Fungi

Product type

Yeast

Strain designation

52 [52D, CDC B3179]

Type strain

No

Isolation source

Cerebrospinal fluid

Geographical isolation

United States; Maryland

Applications

Agricultural research

Product format

Freeze-dried

Storage conditions

2°C to 8°C

General

Specific applications

Biomedical Research and Development Material

Emerging infectious disease research

Preceptrol

No

Characteristics

Serotype

D

Comments

Phenotypic switching

Antibody response to capsular glucuronoxylomannan, GXM, in mice

Experimental infection in rats

Facultative intracellular pathogen

Crystal formation

Immunoglobulin M specificity to epitopes

Induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta in human microglia

Interaction between lung surfactants and alveolar macrophages in defense against Cryptococcus neoformans

Melanization decreases the suseptibility of Cryptococcus neoformans to enzymatic degradation.

Multilocus enzyme typing

Handling information

Medium

ATCC Medium 200: YM agar or YM broth

ATCC Medium 28: Emmons' modification of Sabouraud's agar/broth

ATCC Medium 336: Potato dextrose agar (PDA)

Temperature

37°C

Atmosphere

Aerobic

Handling procedure

For freeze-dry (lyophilized) ampoules:

Open an ampoule according to enclosed instructions.

From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet.  Stir to form a suspension.

Aseptically transfer the suspension back into the test tube of sterile distilled water.

Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.

Mix the suspension well.  Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.

Incubate the inoculum at the propagation conditions recommended.

Inspect for growth of the inoculum/strain regularly. Viability is typically noticeable after 2-3 days of incubation. However, the time necessary for significant growth will vary from strain to strain.  

Handling notes

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Sequenced data

18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence

GGTTTCCGTAGGTGAACCTGCGGAAGGATCAGTAGAGAATATTGGACTTTGGTCCATTTATCTACCCATCTACACCTGTGAACTGTTTATGTGCTTCGGCACGTTTTACACAAACTTCTAAATGTAATGAATGTAATCATATTATAACAATAATAAAACTTTCAACAACGGATCTCTTGGCTTCCACATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCAACTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGAGTCATGAAAATCTCAATCCCTCGGGTTTTATTACCTGTTGGACTTGGATTTGGGTGTTTGCCGCGACCTGCAAAGGACGTCGGCTCGCCTTAAATGTGTTAGTGGGAAGGTGATTACCTGTCAGCCCGGCGTAATAAGTTTCGCTGGGCCTATGGGGTAGTCTTCGGCTTGCTGATAACAACCATCTCTTTTTGTTTGACCTCAAATCAGGTAGGGCTACCCGCTGAACTTAAGCATATCAATAA

D1D2 region of the 26S ribosomal RNA gene

ATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGTGAACCGGGAAGAGCTCAAATTTGAAATCTGGCGTCCTCCGGGCGTCCGAGTTGTAATCTACAGAAACGTTTTCCGTGCTGGACCGTGTCTAAGTCCCTTGGAATAGGGTATCAAAGAGGGTGACAATCCCGTACTTGACACGATCACCAGTGCTCTGTGATACGTTTTCTACGAGTCGCGTTACTTGGGAGTGTAGCGCAAAATGGGTGGTAAACTCCATCTAAAGCTAAATATTGGTGGAAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCGTGTCTATTGGGTTCAGCCAGTTCTGCTGGTGTATTCCCTTTAGACGGGTCAACATCAGTTCTGATCGGTGGATAAGGGCTGGAGGAATGTGGCACTCTTCGGGGTGTGTTATAGCCTCCTGTCGCATACACTGGTTGGGACTGAGGAATGCAGCTCGCCTTTATGGCCGGGGTTCGCCCACGTTCGAGCTTAGGATGTTGACAAAATGGCTTTAAACGAC

History

Deposited as

Cryptococcus neoformans (Sanfelice) Vuillemin

Synonyms

Cryptococcus neoformans var. shanghaiensis Liao, Torula histolytica Stoddard et Cutler, Cryptococcus hominis Vuillemin

Depositors

JE Bennett

Type of isolate

Human

Cross references

GenBank L05428 17S rRNA gene, complete gene

GenBank L14068 5.8S and 25S ribosomal RNA sequences

GenBank L27078 5S ribosomal RNA and intergenic spacer

GenBank AF032431 orotate phosphoribosyltransferase (URA5) gene, complete coding sequence

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.