平台编号:Bio-137451
Shewanella algae Simidu et al. emend. Nozue
51181 ™
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Product category
Bacteria
Strain designation
BrY
Type strain
No
Genome sequenced strain
Yes
Isolation source
Sediment from Great Bay estuary
Geographical isolation
United States; New Hampshire
Product format
Freeze-dried
Storage conditions
2°C to 8°C
General
Specific applications
Reduces iron (ferric, FeIII) ferric iron
Resistant to chromium
Biotechnology
Preceptrol
No
Handling information
Medium
ATCC Medium 2: Marine agar 2216 or marine broth 2216
Temperature
30°C
Atmosphere
Aerobic
Handling procedure
Open vial according to enclosed instructions or visit www.atcc.org for instructions.
Rehydrate the entire pellet with approximately 0.5 mL of #2 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #2 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
Use several drops of the primary broth tube to inoculate a #2 plate and/or #2 agar slant.
Incubate at 30°C for 24 hours.
Handling notes
To demonstrate anaerobic growth by dissimilatory iron (III) reduction, use ATCC Medium #1931 broth.
1931 Broth: Ferric citrate Medium
Ferric citrate, 13.7 g
NaHCO3, 2.5 g
KCl, 0.1 g
NH4Cl, 1.5 g
NaH2PO4, 0.6 g
Wolfe's Vitamin Solution (see below), 10.0 mL
Wolfe's Mineral Solution (see below), 10.0 mL
Sodium lactate, 30.0 mM
Distilled water, 1.0 L
Boil ferric citrate in distilled water to dissolve; cool to room temperature. Adjust to pH 6.6 with 10N NaOH. Add remaining components. Equilibrate medium under 80% N2, 20% CO2. Dispense medium anaerobically under same gas phase. Autoclave anaerobically at 121oC for 15 minutes. Final pH 7.0.
Wolfe's Vitamin Solution:
Biotin, 2.0 mg
Folic acid, 2.0 mg
Pyridoxine HCl, 10.0 mg
Thiamine HCl, 5.0 mg
Riboflavin, 5.0 mg
Nicotinic acid, 5.0 mg
Calcium D-(+)-pantothenate, 5.0 mg
Vitamin B12, 0.1 mg
p-Aminobenzoic acid, 5.0 mg
Thioctic acid, 5.0 mg
Distilled water, 1.0 L
Wolfe's Mineral Solution:
Nitrilotriacetic acid, 1.5 g
MgSO4.7H2O, 3.0 g
MnSO4.H2O, 0.5 g
NaCl, 1.0 g
FeSO4.7H2O, 0.1 g
CoCl2.6H2O, 0.1 g
CaCl2, 0.1 g
ZnSO4.7H2O, 0.1 g
CuSO4.5H2O, 0.01 g
AlK(SO4)2.12H2O, 0.01 g
H3BO3, 0.01 g
Na2MoO4.2H2O, 0.01 g
Distilled water, 1.0 L
Add nitrilotriacetic acid to approximately 500 mL of water and adjust to pH 6.5 with KOH to dissolve the compound. Bring volume to 1.0 L with remaining water and add remaining compounds one at a time.
Whole-genome Sequencing
History
Deposited as
Shewanella algae Simidu et al. emend. Nozue
Depositors
F Caccavo
Type of isolate
Environmental
Cross references
GenBank AF005691 Shewanella alga DNA gyrase beta-subunit (gyrB) gene, partial cds.
Legal disclaimers
Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
Disclaimers
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
企业名称
北京百欧博伟生物技术有限公司
企业信息已认证
企业类型
个企
信用代码
110108015577306
成立日期
2013-01-24
注册资本
500
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