平台编号:Bio-135423
Human herpesvirus 5
VR-3348™
Human herpesvirus 5 strain UL32-EGFP-HCMV-TB40 is propagated in MRC-5 cells (ATCC CCL-171). This strain was sourced from the in vitro recombination of HCMV strain TB40 with a plasmid carrying the TB40 UL32 gene fused to GFP. Because GFP is associated with a structural protein, virus particles fluoresce green under appropriate illumination; this is useful for following intracellular traffic of virus particles.
Product category
Viruses
Product type
Animal virus
Classification
Herpesviridae, Cytomegalovirus, Human betaherpesvirus 5
Strain designation
UL32-EGFP-HCMV-TB40
Common name
Cytomegalovirus
Isolation source
In vitro recombination of HCMV strain TB40 with a plasmid carrying the TB40 UL32 gene fused to GFP.
Applications
Infectious disease research
Product format
Frozen
Storage conditions
-70°C or colder
General
Specific applications
Tracking intracellular traffic of virus particles.
Characteristics
Comments
Recombinant HCMV virus with GFP fused to the C terminus of the tegument phosphoprotein pp150, the product of the UL32 gene. Because GFP is associated with a structural protein, virus particles fluoresce green under appropriate illumination. Useful for following intracellular traffic of virus particles.
Handling information
Host
MRC-5 (ATCC CCL-171)
Effects
CPE; cell rounding; cell enlargement
Complete medium
EMEM (ATCC 30-2003) supplemented with 2% FBS (ATCC 30-2020)
Temperature
37°C
Incubation
7-21 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Recommendation for infection
Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (eg, 1 mL per 25 cm2) diluted to provide an optimal MOI (eg, 0.1 to 1.0). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Handling notes
Recombinant HCMV virus with GFP fused to the C terminus of the tegument phosphoprotein pp150, the product of the UL32 gene. Because GFP is associated with a structural protein, virus particles fluoresce green under appropriate illumination. Useful for following intracellular traffic of virus particles.
Key abbreviations
°C, Degrees Celsius
CO2, Carbon dioxide
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum
MOI, Multiplicity of infection
Quality control specifications
History
Legal disclaimers
企业名称
北京百欧博伟生物技术有限公司
企业信息已认证
企业类型
个企
信用代码
110108015577306
成立日期
2013-01-24
注册资本
500
经营范围
技术开发、技术推广、技术转让、技术咨询、技术服务;销售机械设备、电子产品、计算机、软件及辅助设备、五金交电(不得从事实体店铺经营)、通讯设备、建筑材料(不得从事实体店铺经营)、医疗器械(限一类)、化工产品(不含危险化学品)。(企业依法自主选择经营项目,开展经营活动;依法须经批准的项目,经相关部门批准后依批准的内容开展经营活动;不得从事本市产业政策禁止和限制类项目的经营活动。)
北京百欧博伟生物技术有限公司
公司地址
北京市顺义区信中北街16号院3号楼4层432
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