Anti-beta I Tubulin antibody
种属反应性Human,Mouse,Rat
验证应用WB,IHC-P,FC
抗体类型小鼠单抗
免疫原KLH conjugated synthetic peptide derived from human beta I Tubulin
偶联Non-conjugated
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein G
亚细胞定位Cytoplasm, cytoskeleton
其它名称
2810484G07Rik
Beta tubulin 1, class VI
Class VI beta tubulin
WB:1:5000-20000
IHC-P:1:400-800
FC:1:100-500
Fig1: Sample:
K562 Cell (Human) Lysate at 40 ug
Jurkat Cell (Human) Lysate at 40 ug
Primary: Anti- beta I Tubulin at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.
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企业名称
上海泽叶生物科技有限公司
企业信息已认证
企业类型
信用代码
91310116MAJ8N934E
成立日期
2016-08-09
注册资本
100
经营范围
生物科技
上海泽叶生物科技有限公司
公司地址
上海市松江区国家经济开发区北松公路5629号聚科生物松江园区
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