pUAST果蝇编辑质粒
基本信息
原核抗性: 氨苄青霉素Amp
质粒简介
pUAST consists of five tandemly arrayed optimized GAL4 binding sites followed by the hsp70 TATA box and transcriptional start , a polylinker containing unique restriction sites for EcoRI, BglII, NotI, Xho, KpnI and XbaI and the SV40 small T intron and polyadenylation site. These features are included in a P-element vector (pCaSpeR3)containing the P element ends (P3' and P5') and the white gene which acts as a marker for successful incorporation into the Drosophila genome. More information: Brand and Perimon (1993) Development 118 401-415 P element-based vector for Gal4-regulated expression of genes in Drosophila.
质粒图谱![pUAST.png pUAST.png](https://img1.17img.cn/17img/images/201908/uepic/d03aa88e-3b45-4e55-a891-742cdb0009c8.jpg)
pUAST果蝇编辑质粒使用说明:
1、收到质粒干粉后请先5000rpm离心1min,再加入20μl无菌水溶解质粒,室温放置1min;
2、从-80℃冰箱中取出相应的感受态,置于冰盒上解冻,并做好标记;
3、取2μl质粒加至100μl感受态中,冰浴30min;
4、42℃热激90s,再冰浴2min;
5、加入900μl无抗的LB液体培养基,180rpm震荡培养45min;
6、6000rpm离心5min,仅留100ul上清混匀菌体沉淀;
7、混匀后的菌液加至对应抗性的LB平板上,倒入适量玻璃珠,涂匀液体;
8、将平板正向培养1h,再倒置培养12h~16h;
9、挑取单克隆菌落至对应抗性的LB液体培养基中,震荡培养12h~16h,根据实验需要提取质粒。
pUAST果蝇编辑质粒注意事项:
1、如果您收到的是甘油菌种,请先四区划线,挑取单克隆培养。
2、如果第二天转化平板长的过多,请将质粒按比例稀释后再转化。
pUAST果蝇编辑质粒信息由上海钦诚生物科技有限公司为您提供,如您想了解更多关于pUAST果蝇编辑质粒报价、型号、参数等信息,欢迎来电或留言咨询。除供应pUAST果蝇编辑质粒外,上海钦诚生物科技有限公司还可为您提供TMD8 人弥漫性大B细胞淋巴瘤 QCH818、SNU638 人胃癌细胞 QCH815、IDG-CM6 人牙骨质细胞 QCH803等产品,公司有专业的客户服务团队,是您值得信赖的合作伙伴。