英文名称 phospho-CHK1 (Ser286)
中文名称 磷酸化细胞周期检测点激酶1抗体
别 名 Chk1 (phospho S286); p-Chk1 (phospho S286); Chk1 (Phospho-Ser286); CHEK1 (phospho Ser286); CHEK1 (phospho Ser286); p-CHEK1 (Ser286); Cell cycle checkpoint kinase; Checkpoint, S. pombe, homolog of, 1; Checkpoint kinase 1; Checkpoint kinase 1 homolog (S. pombe); CHEK 1; CHEK-1; CHEK1; Chk 1; Chk1; CHK1 checkpoint homolog (S. pombe); EC 2.7.11.1; rad27; Serine/threonine protein kinase Chk1; Serine/threonine-protein kinase CHK1; STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae); C85740; CHK1_HUMAN.
说 明 书 0.1ml
产品类型 磷酸化抗体
研究领域 细胞生物 染色质和核信号 信号转导 细胞周期蛋白 激酶和磷酸酶 表观遗传学
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Chicken, Dog, Horse,
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 磷酸化细胞周期检测点激酶1抗体(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 54kDa
细胞定位 细胞核 细胞浆
性 状 Lyophilized or Liquid
浓 度 1mg/1ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from mouse CHEK1 around the phosphorylation site of Ser286.
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
磷酸化细胞周期检测点激酶1抗体产品介绍 background:
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2011].
Function:
Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Also phosphorylates NEK6. Binds to and phosphorylates RAD51 at 'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, thereby promoting DNA repair by homologous recombination. Phosphorylates multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation. Also promotes repair of DNA cross-links through phosphorylation of FANCE. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may enhance chromatin assembly both in the presence or absence of DNA damage. May also play a role in replication fork maintenance through regulation of PCNA. May regulate the transcription of genes that regulate cell-cycle progression through the phosphorylation of histones. Phosphorylates histone H3.1 (to form H3T11ph), which leads to epigenetic inhibition of a subset of genes. May also phosphorylate RB1 to promote its interaction with the E2F family of transcription factors and subsequent cell cycle arrest. Isoform 2: Endogenous repressor of isoform 1, interacts with, and antagonizes CHK1 to promote the S to G2/M phase transition.
磷酸化细胞周期检测点激酶1抗体
hz-1270 Cyclin-D3(Mouse Cyclin-D3) ELISA Kit 小鼠细胞周期素D3
hz-1270 CK-18(Human cytokeratin 18) ELISA Kit 人细胞角蛋白18
hz-1271 Cyclin-D2(Mouse Cyclin-D2) ELISA Kit 小鼠细胞周期素D2
hz-1271 CgA(Human Chromogranin) ELISA Kit 人嗜铬蛋白A
hz-1271 LTC4 ELISA Kit 大鼠白三烯C4
hz-1272 Cyt-C ELISA Kit 大鼠细胞色素C
hz-1272 pRB(Human retinoblastoma tumor suppressor protein) ELISA Kit 人视网膜母细胞瘤抑制蛋白
hz-1272 E(Mouse estrogen) ELISA Kit 小鼠雌激素
hz-1273 Ang- I ELISA Kit 大鼠血管紧张素Ⅰ
hz-1273 GRO Alpha/CXCL1/MGSA(Human growth-regulated oncogene Alpha/melanoma growth stimulating activity) ELISA Kit 人生长调节致癌基因α/黑素瘤生长刺激因子
hz-1273 Cyclin-D1(Mouse Cyclin-D1) ELISA Kit 小鼠细胞周期素D1
hz-1274 MBP(Mouse myelin basic protein) ELISA Kit 小鼠髓磷脂碱性蛋白
hz-1274 MPF(Human maturation promoting factor) ELISA Kit 人成熟促进因子
hz-1274 MC Ab ELISA Kit 大鼠黑色素细胞抗体
hz-1275 TGF- Alpha(Mouse transforming growth factor Alpha) ELISA Kit 小鼠转化生长因子α
hz-1275 ASGPR(Human asialoglyco protein receptor) ELISA Kit 人去唾液酸糖蛋白受体
hz-1275 DPP IV ELISA Kit 大鼠二肽基肽酶Ⅳ
hz-1276 C4a ELISA Kit 大鼠过敏毒素/补体片断4a
hz-1276 sTfR(Human soluble transferrin receptor) ELISA Kit 人可溶性转铁蛋白受体
hz-1276 AFP(Mouse Alpha-fetoprotein) ELISA Kit 小鼠甲种胎儿球蛋白/甲胎蛋白
hz-1277 MT(Human Metallothionein) ELISA Kit 人金属硫蛋白
hz-1277 PAP(Mouse Prostatic Acid Phosphatase) ELISA Kit 小鼠前列腺酸性磷酸酶
hz-1277 C5a ELISA Kit 大鼠补体片断5a
hz-1278 C3a ELISA Kit 大鼠补体片断3a
hz-1278 PSA(Mouse prostate specific antigen) ELISA Kit 小鼠前列腺特异性抗原
hz-1278 AFP-L3(Human alpha-fetoprotein Lens culinaris agglutiin 3) ELISA Kit 人小扁豆素结合型甲胎蛋白/甲胎蛋白异质体3
hz-1279 AFP-L2(Human alpha-fetoprotein Lens culinaris agglutiin 2) ELISA Kit 人小扁豆素结合型甲胎蛋白/甲胎蛋白异质体2
hz-1279 FS ELISA Kit 大鼠卵泡抑素
hz-1279 (Mouse ovarian cancer marker-CA125) ELISA Kit 小鼠卵巢癌标志物CA125
hz-1280 HD ELISA Kit 大鼠组织蛋白去乙酰化酶
hz-1280 AFP-L1(Human alpha-fetoprotein Lens culinaris agglutiin 1) ELISA Kit 人小扁豆素结合型甲胎蛋白/甲胎蛋白异质体1
hz-1280 (Mouse mammary carcinoma Marker-CA153) ELISA Kit 小鼠乳腺癌标志物-CA153
hz-1281 (Mouse Gastrointestinalcancer Marker-CA199) ELISA Kit 小鼠胃肠癌标志物CA199
hz-1281 MART/Melan-A(Human Melanoma Marker A) ELISA Kit 人黑色素瘤标记物
hz-1281 E-Selectin/CD62E ELISA Kit 大鼠E选择素
磷酸化细胞周期检测点激酶1抗体 信息由上海沪震生物科技有限公司为您提供,如您想了解更多关于磷酸化细胞周期检测点激酶1抗体 报价、型号、参数等信息,欢迎来电或留言咨询。除供应磷酸化细胞周期检测点激酶1抗体 外,上海沪震生物科技有限公司还可为您提供HSP27 (Ab-15) 抗体、ASK1 (Ab-83) 抗体、p21Cip1 (Ab-145) 抗体等产品,公司有专业的客户服务团队,是您值得信赖的合作伙伴。