磷酸化细胞周期检测点激酶2抗体

英文名称    Phospho-CHK2 (Thr68)    

中文名称    磷酸化细胞周期检测点激酶2抗体    

别    名    bA444G7; CHK2 checkpoint homolog; CHK2_HUMAN; Serine/threonine-protein kinase Chk2; CDS 1; CDS1; Checkpoint kinase 2; Checkpoint like protein CHK2; Chek 2; Chek2; Chk 2; CHK2 checkpoint homolog (S. pombe); CHK2 checkpoint homolog; HuCds 1; HuCds1; LFS 2; LFS2; PP1425; RAD 53; RAD53; Rad53 homolog; Serine/threonine protein kinase Chk2.    

说 明 书    0.1ml      

产品类型    磷酸化抗体     

研究领域    肿瘤  染色质和核信号  激酶和磷酸酶  表观遗传学      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Dog, Pig, Cow, Horse,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 磷酸化细胞周期检测点激酶2抗体（石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    61kDa    

细胞定位    细胞核     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated Synthesised phosphopeptide derived from human CHK2 around the phosphorylation site of Thr68    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

磷酸化细胞周期检测点激酶2抗体产品介绍    background:

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Function:
Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells.    

磷酸化细胞周期检测点激酶2抗体

hz-6124R p95 NBS1/Cell cycle regulatory protein p95  细胞周期调节蛋白P95抗体

hz-6125R phospho-p95 NBS1(Ser343)  磷酸化滑膜细胞凋亡抑制物1抗体

hz-6126R FAM123B/AMER1  肾母细胞瘤X蛋白抗体

hz-6127R KARS2/Lysyl tRNA synthetase  赖氨酸tRNA的连接酶抗体

hz-6128R WSTF  转录因子WSTF抗体

hz-6129R Wnt8A  信号通路WNT8A抗体

hz-6133R WNT2  信号通路Wnt2抗体

hz-6137R CtIP/RBBP8  视网膜母细胞瘤结合蛋白8抗体

hz-6138R EID1  乙酰基转移酶EID1抗体

hz-6139R KDM5B/PLU1/Jarid1B  组蛋白去甲基化酶JARID1B抗体

hz-6140R KMT8/Riz1/Riz2  转录因子GATA3结合蛋白G3B抗体

hz-6141R GLIPR1  脑胶质瘤发病机制相关蛋白1抗体

hz-6142R RING1  环指蛋白1抗体

hz-6143R PIG3/TP53I3  p53诱导蛋白3抗体

hz-6144R PRMT4  组蛋白精氨酸甲基转移酶CARM1抗体

hz-6145R TP53I13  肿瘤蛋白p53诱导蛋白13(肿瘤抑制基因)

hz-6146R p53 DINP1  p53诱导核蛋白1抗体

hz-6147R p73 alpha  p53相关蛋白P73α抗体

hz-6148R Frizzled 1/Wnt receptor  Wnt信号受体蛋白抗体

hz-6149R GNAT3  G蛋白转录因子α3抗体

hz-6150R GPR124  G蛋白偶联受体124抗体（肿瘤血管内皮标记物）

hz-6151R GPR172B  G蛋白偶联受体172B抗体

hz-6152R GNAQ/Gα q  G蛋白α亚单位蛋白Q抗体

hz-6155R RAP1GDS1  G蛋白GTP-GDP解离刺激因子1抗体

hz-6156R RASGEF1A  鸟嘌呤核苷酸交换因子rasgef1a抗体

hz-6157R RAB4  癌基因RAS相关蛋白Rab4A抗体

hz-6158R NSP1/SH2D3A  SH2结构域蛋白3A抗体

hz-6159R STARD8  GTP酶激活因子STARD8抗体

hz-6161R WASF3  Verprolin同源结构域包含蛋白3抗体

hz-6162R ARL3  ADP核糖基化样因子ARL3抗体

hz-6163R DIRAS1  RAS相关抑细胞生长蛋白1抗体