Animal-Free Recombinant Human IL-1alpha

IL-1α is a non-secreted, proinflammatory cytokine produced in a variety of cells, including monocytes, tissue macrophages, keratinocytes, and other epithelial cells. Both IL-1α and IL-1β bind to the same receptor and have similar, if not identical, biological properties. These cytokines have a broad range of activities including the stimulation of thymocyte proliferation by inducing IL-2 release, Bcell maturation and proliferation, mitogenic FGF-like activity and the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine.  Recombinant Human IL-1α is an 18.0 kDa protein containing 159 amino acid residues.

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte, Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF)

AA Sequence: SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to stimulate the proliferation of mouse D10S cells. The expected ED₅₀ is ≤0.001 ng/ml, corresponding to a specific activity of ≥1 x 10⁹ units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 18 kDa

Accession Number: P01583

Gene ID: 3552

Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug)