Animal-Free Recombinant Human/Murine/Rat Activin A (E.coli derived)

Activin A is a TGF-β family member that exhibits a wide range of biological activities, including regulation of cellular proliferation and differentiation, and promotion of neuronal survival.  Elevated levels of Activin A in human colorectal tumors and in postmenopausal women have been implicated in colorectal and breast cancers, respectively.  The biological activities of Activin A can be neutralized by inhibins and by the diffusible TGF-β antagonist, follistatin.  Activin A binds to the two forms of activin receptor type I (Act RI-A and Act RI-B) and two forms of activin receptor type II (Act RII-A and Act RII-B).  Activins are homodimers or heterodimers of different β subunits.  They are produced as precursor proteins with an amino terminal propeptide that is cleaved to release the C-terminal bioactive ligand.  Recombinant Human/Murine/Rat Activin A is a 26.0 kDa disulfide-linked homodimer of two βA chains, each containing 116 amino acid residues.

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: Inhibin beta-1, FRP, FSH (Follicle-stimulating hormone)-releasing protein, Erythroid Differentiation Factor (EDF)

AA Sequence (monomer): GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS

Purity: ≥ 97% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to inhibit the proliferation of mouse MPC-11 cells. The expected ED₅₀ is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 10⁵ units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 26 kDa

Accession Number: P08476

Gene ID: 3624

Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug)

Note:

1mg will be provided as 2x500μg.