Members of the Hedgehog (Hh) family are highly conserved proteins that are widely represented throughout the animal kingdom. The three known mammalian Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh), are structurally related, and share a high degree of amino acid sequence identity (e.g. Shh and Ihh are 93% identical). The biologically active form of each Hh molecule is obtained by autocatalytic cleavage of their precursor proteins, and each corresponds to approximately one half of the N-terminal portion of the precursor molecule. Although Hh proteins have unique expression patterns and distinct biological roles within their respective regions of secretion, they use the same signaling pathway and can be substituted for one another in experimental systems. Recombinant E. coli-derived Human Sonic Hedgehog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the naturally occurring, chemically modified, Cys residue.
Source: E.coli
Synonyms: Hhg-1
AA Sequence: IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity: Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.8-1.0 μg/ml.
Recombinant Human Sonic Hedgehog (Shh) Biological Activity Graph
Calculated Molecular Weight: 20 kDa
Accession Number: Q15465
Gene ID: 6469
Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)
Note:
1mg will be provided as 2x500μg.
Cross Reactivity Cited in References:
Human, Mouse
Recombinant Human Sonic Hedgehog (Shh)信息由上海远慕生物科技有限公司为您提供,如您想了解更多关于Recombinant Human Sonic Hedgehog (Shh)报价、型号、参数等信息,欢迎来电或留言咨询。除供应Recombinant Human Sonic Hedgehog (Shh)外,上海远慕生物科技有限公司还可为您提供维生素B6测试盒 微量法、类胡萝卜素(carotenoid)含量测试盒 分光光度法、共价结合型果胶(CSP)含量测试盒 微量法等产品,公司有专业的客户服务团队,是您值得信赖的合作伙伴。