果聚糖（HK法）测试剂盒 ZZK-K-FRUCHK

果聚糖（HK法）测试剂盒 ZZK-K-FRUCHK

产品货号：ZZK-K-FRUCHK

产品品名：果聚糖（HK法）测试剂盒

英文品名：Fructan HK Assay Kit

规格型号：50 assays

果聚糖是β-D-呋喃果糖的多聚体。是由果糖聚合而生成的多糖的总称。果糖残基数目一般为7～35,少数为90～260。分3种类型:①菊糖型。果糖残基以β2→1糖苷键连结而成的线形分子。②左聚糖型。果糖残基以β2→6糖苷键连结而成的线形分子。③混合型。果聚糖用作增稠剂、稳定剂、胶凝剂、黏结剂。用于一般加工食品。常作为乳化稳定剂、气泡稳定剂使用。

上海甄准生物提供果聚糖（HK法）测试剂盒，采用紫外分光光度计法（340nm）测定食品、饮料以及其他原料中的果聚糖。

原理：

总检测时间：90min

检测限：样品重量的1-100%

适用样品：面粉、植物原料（如洋葱）、食品以及其他原料

优点：

l  价格低廉

l  所有试剂配制后的稳定性>12个月

l  方法简单

l  包含标准品

l  该方法是AOAc方法999.03和AACC方法32-32的改良方法

REFERENCES:

1. Lewis, D. H. (1993). “Nomenclature and diagrammatic representation of oligomeric fructans - a paper for discussion.” New Phytologist, 124, 583-594.

2. McCleary, B. V. and Blakeney, A. B. (1999). “Measurement of inulin and oligofructan.” Cereal Foods World, 44, 398-406.

3. Pontis, H. G. (1966). “Observations on the de novo synthesis of fructosans in vivo.” Arch. Biochem. Biophys., 116, 416-423.

4. Pontis, H. G. (1990). “Fructans.” In Methods in Plant Biochemistry (P. M. Dey and J. B. Harborne, Eds.). Academic Press, New York, pp 353-369.

5. Quemener, B., Thibault, J. -F. and Coussement, P. (1993). “Determination of inulin and oligofructose in food products, and integration in the AOAC method for measurement of total dietary fibre.” Lebansm.-Wiss. u. Technol., 27, 125-132.6.

6. Uchiyama, T. (1993). “Metabolism in microorganisms. Part II. Biosynthesis and degradation of fructans by microbial enzymes other than levansucrase.” In Science and technology of fructans

(M. Suzuki and N. J. Chatterton, Eds.). CRC Press, pp 170-190.

7. McCleary, B. V, Murphy, A. and Mugford, D. C. (2000). “Determination of Oligofructans and Fructan Polysaccharides in Foodstuffs by an Enzymatic/Spectrophotometric Method: Collaborative Study.” J. AOAC International, 83, 356-364.

8. McCleary, B. V. and Rossiter, P. (2004). “Measurement of novel dietary fibres.” J. AOAC International, 87, 707-711.

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