Eukaryotic Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)
核因子κB受体激活因子配体(RANkL)真核蛋白
[ PROPERTIES ]
Source: Eukaryotic expression
Host: 293F Cell
Residues: Arg72~Asp316
Tags: N-terminal His Tag
Subcellular Location: Membrane, Secreted, Cytoplasm
Purity: > 95%
Traits: Freeze-dried powder
Buffer formulation:PBS, pH7.4, containing 5% trehalose.
Original Concentration: 100µg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 7.1
Predicted Molecular Mass: 29.2kDa
Accurate Molecular Mass: 35kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in ddH2O to a concentration of 0.1-0.3 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.
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