谷丙转氨酶(SGPT)检测检测原理:试剂盒采用双抗体一步夹心法酶联免疫吸附试验(ELISA)。往预先包被大鼠谷胱甘肽-过氧化物酶(GSH-PX)获抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并彻底洗涤谷丙转氨酶(SGPT)检测用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的大鼠谷胱甘肽-过氧化物酶(GSH-PX)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算样品浓度。
谷丙转氨酶(SGPT)检测需要提供的试剂和器材
37℃恒温箱
标准规格酶标仪
精密移液器及一次性吸头
蒸馏水
一次性试管
吸水纸
谷丙转氨酶(SGPT)检测按试剂组成分类
一、单试剂
①优点:操作简便适用于各类生化分析,节约试剂位。
②缺点:配方复杂(稳定剂),稳定性,不能完全避免内外源物质干扰。
二、双试剂
①优点:可较彻底排除样品空白和内外源干扰物,试剂稳定,便于储存,运输和使用,可用抑制法直接测定某些同工酶。
②缺点:多占试剂位。
更多产品引荐:?B-02144 ?Anti-CD71/TFR(Transferrin Receptor)
?B-02145 ?Anti-CD73(5′-nucleotidase cytosolicⅡ)
?B-02146 ?Anti-CD79A/IGBP-1(Immunoglobuin binding protein-1)
?B-02147 ?Anti-CD82/KAI1
?B-02148 ?Anti-CD8(mouse monoclonal antibody)
?B-02149 ?Anti-CD86/B7-2
?B-02150 ?Anti-Cdc2/P34CDC2/CDK1(Cell division control protein 2)
?B-02151 ?Anti-CD335/NKp46/NCR1
?B-02152 ?Anti-CD336/NKp44/NCR2(Natural killer cell p44-related protein)
?B-02153 ?Anti-CD337/NKp30/NCR3(Natural killer cell p30-related protein)
?B-02154 ?Anti-Cdc6(Cell Division Cycle protein 6)
?B-02155 ?Anti-CD339/Jagged1
?B-02156 ?Anti-Cdc34(Cell Division Cycle protein 34)
?B-02157 ?Anti-CDK1 (Cyclin-Dependent Kinase 1)
?B-02158 ?Anti-CDK2 (Cyclin-Dependent Kinase 2)
?B-02159 ?Anti-CDK3 (cyclin-dependent kinase 3)
?B-02160 ?Anti-CDK4 (cyclin-dependent kinase 4)
?B-02161 ?Anti-CDK5(Cyclin Dependent Kinase 5)
?B-02162 ?Anti-CDK6 (Cyclin Dependent Kinase 6)
?B-02163 ?Anti-CDK7/Cyclin H/MAT1
?B-02164 ?Anti-CDK8(Cyclin Dependent Kinase 8)
?B-02165 ?Anti-CCNT1/CDK9 (Cyclin Dependent Kinase 9)
?B-02166 ?Anti-Cdkn1c/p57/Kip2 (Cyclindependent kinase inhibitor 1C)
?B-02167 ?Anti-CGP-39/YKL-40(Chitinase-3-like protein 1 )
?B-02168 ?Anti-CEA (Carcinoembryonic Antigen )
?B-02169 ?Anti-CEA(mouse monoclonal)
?B-02170 ?Anti-CEA(mouse monoclonal)
?B-02171 ?Anti-CEAcam8/CD67 (carcinoembryonic Anti-gen-related cell adhesion molecule 8)
A-01000 ?Anti-GAPDH(mouse Anti-GAPDH Monoclonal)
A-01001 ?Anti-β-Actin
A-01002 ?Anti-14-3-3 protein
谷丙转氨酶(SGPT)检测信息由上海信裕生物科技有限公司为您提供,如您想了解更多关于谷丙转氨酶(SGPT)检测报价、型号、参数等信息,欢迎来电或留言咨询。除供应谷丙转氨酶(SGPT)检测外,上海信裕生物科技有限公司还可为您提供OVA Conjugated Leucine (Leu) XY95654CPSGe、Recombinant Glial Fibrillary Acidic Protein (GFAP) XY92135RPMu03、Recombinant Carcinoembryonic Antigen (CEA) XY90800RPHu01等产品,公司有专业的客户服务团队,是您值得信赖的合作伙伴。