格雷斯/奥泰(Grace/Alltech):Alltima? HP:极其稳定耐用的高性能色谱柱, 键和相流失极低,尤其适合LCMS 使用
1:更好的峰形-高纯硅胶基质消除峰拖尾问题
2:更长的柱寿命-极强的耐用性,减少停机时间和使用成本
3:更小的键和相流失-极低或测不到键和相流失,特别适用于LCMS 或ELSD 检测
4:多种键和相和柱规格-为您优化保留、分离和分析时间提供各种选择
Alltima? HP系列是我们在高纯硅胶上使用了最好的键和相技术得到的产品。这个系列有各种不同的键和相供选择,稳定耐用,性能卓越,特别适用于高难样品的分析。当您使用的是较容易流失的键和相类型如C18 Amide(C18 酰胺基)或Cyano(氰基)时,或者使用的冲洗条件较为苛刻(酸碱性较强,或柱温较高),或者配用的检测器对于键和相流失较为敏感如MS 或ELSD 时,也应该优先选择Alltima? HP以获得更好更稳定的使用效果。
Alltima? HP键和相规格
|
键和相
| 填料基质
| 填料形状
| 填料粒径
| 孔径
| 表面积
| 碳载量
| 键和类型
| 封尾?
| USP代码
|
C18
| 硅胶
| 球形
| 3, 5μm
| 190A
| 200m2/g
| 12%
| 单点型
| 是
| L1
|
C18 EPS
| 硅胶
| 球形
| 3, 5μm
| 190A
| 200m2/g
| 4%
| 单点型
| 是
| L1
|
C18 HiLoad
| 硅胶
| 球形
| 3, 5μm
| 100A
| 450m2/g
| 24%
| 单点型
| 是
| L1
|
C18 AQ
| 硅胶
| 球形
| 3, 5μm
| 100A
| 450m2/g
| 20%
| 单点型
| 是
| L1
|
C18 Amide
| 硅胶
| 球形
| 3, 5μm
| 190A
| 200m2/g
| 12%
| 单点型
| 是
| L1
|
C8
| 硅胶
| 球形
| 3, 5μm
| 190A
| 200m2/g
| 8%
| 单点型
| 是
| L7
|
Cyano (CN)
| 硅胶
| 球形
| 3, 5μm
| 190A
| 200m2/g
| 4%
| 单点型
| 是
| L10
|
Silica
| 硅胶
| 球形
| 3, 5μm
| 100A
| 450m2/g
| ――
| ――
| 无 | L3
|
HILIC
| 硅胶
| 球形
| 1.5, 3, 5μm
| 120A
| 230m2/g
| ――
| ――
| ――
| L3
|
![](http://www.taixinbio.com/upload/image/20150306221703_72403.jpg)
在中性pH7 的条件下检测碱性很强的药物,依然能得到对称性很好的峰形。
![](http://www.taixinbio.com/upload/image/20150306221849_97825.jpg)
Alltima? HP C18 柱显示了pH1 和pH10 条件下极强的稳定性。
pH1 酸性柱寿命测试条件:以乙腈:稀硫酸pH 1.0 (50:50)的混合溶液冲洗柱24,000 倍体积,柱温保持在60 摄氏度。最上方蓝色线条谱图为经
受酸性条件冲洗后的测试谱图。pH10 碱性柱寿命测试条件:以乙腈:氨水 pH10 (50:50)的混合溶液冲洗柱24,000 倍体积,柱温保持在20 摄氏
度。次上方红色线条谱图为经受碱性条件冲洗后的测试谱图。两谱图与测试进行前的初始谱图(最下方的黑色线条谱图)几乎相当。
![](http://www.taixinbio.com/upload/image/20150306221945_95126.jpg)
测试Alltima? HP柱和其他柱在MS 检测器上显示的键和相流失情况。最左边是未接入柱时的空白值0.82,Alltima? HP Silica,C18 和C18 Hiload 柱得到的背景噪音与空白值相当,表明没有可见的键和相流失。市场上其他产品所谓MS 专用柱如“C18 MS 1”和“C18 MS 2”都显示出不同程度的键和相流失(背景噪音高于空白值)。C18 Amide 是 “内嵌”了极性官能团的C18 键和相,容易发生水解,比常规C18 键和相的流失率高,而测试结果表明Alltima HP C18 Amide 的流失也只相当于“C18 MS 2”,远远低于市场上其他C18 Amide 柱的键和相流失程度。
![](http://www.taixinbio.com/upload/image/20150306222058_39334.jpg)
氰基柱是众所周知的易流失、寿命低的色谱柱。柱寿命测试表明Alltima HP 氰基柱比市场上其他各品牌的氰基柱的耐用性好得多。分别以酸性条件和碱性条件考验柱,比较测试前后对样品的保留值变化(保留值降低的程度表示键和相流失的程度)。pH1.0 耐酸稳定性测试条件:用硫酸pH1.0:乙腈 50:50 以1mL/min 持续冲洗柱三天。pH7.5 耐碱稳定性测试条件:用20mM K2HPO4 pH7.5:乙腈 50:50 以1mL/min 持续冲洗柱三天。Alltima?HP 氰基柱的保留值降低仅为2%左右,而其它各品牌氰基柱的保留值降低幅度高达5-30%。
![](http://www.taixinbio.com/upload/image/20150306230912_54714.jpg)
Alltima? HP方法开发套装:
每套含三根不同键和相类型但尺寸相同的色谱柱,请按您的液相系统配置、被测样品类型及复杂程度选择合适的套装。按套购买价格更为优惠。
套装名称
| 柱规格(i.d. x L)
| 柱键和相(及各柱相应货号)
| 货号
|
Kit 1 – C18 mix, 3μm
|
Expedite MS Kit 1
| 2.1 x 20mm
| C18, C18 Hi-Load, C18 Amide (87674, 87692, 87728)
| 87854
|
Analytical Kit 1
| 4.6 x 150mm
| C18, C18 Hi-Load, C18 Amide (87668, 87686, 87722)
| 87850
|
Rocket? Kit 1
| 7 x 53mm
| C18, C18 Hi-Load, C18 Amide (87672, 87690, 87726)
| 87852
|
Kit 2 – Alternate mix, 3μm
|
|
Expedite? MS Kit 2
| 2.1 x 20mm
| C18 Hi-Load, C8, CN (87692, 87746, 87764)
| 87864
|
Analytical Kit 2
| 4.6 x 150mm
| C18 Hi-Load, C8, CN (87686, 87740, 87760)
| 87860
|
Rocket? Kit 2
| 7 x 53mm
| C18 Hi-Load, C8, CN (87690, 87744, 87762)
| 87862
|
Kit 3 – Polar mix, 3μm
|
Expedite? MS Kit 3
| 2.1 x 20mm
| C18 EPS, C18 Amide, CN (87710, 87728, 87764)
| 87874
|
Analytical Kit 3
| 4.6 x 150mm
| C18 EPS, C18 Amide, CN (87704, 87722, 87758)
| 87870
|
Rocket? Kit 3
| 7 x 53mm
| C18 EPS, C18 Amide, CN (87708, 87726, 87762)
| 87872
|
常用Alltima HP 色谱柱和保护柱货号快速获取表:
【有更多规格未列出。如需不同粒径、柱内径或柱长度,请垂询上海宸乔生物科技有限公司】
填料
| 柱硬件规格
| 货号
|
C18, 3μm
| Capillary: 0.150 x 150mm
| 22155
|
| Capillary: 0.300 x 150mm
| 22158
|
| Expedite? MS: 2.1 x 10mm
| 87673
|
| Expedite? MS: 2.1 x 20mm
| 87674
|
| Microbore: 2.1x 50mm
| 87504
|
| Microbore: 2.1 x 150mm
| 87670
|
| Analytical: 4.6 x 50mm
| 87826
|
| Analytical: 4.6 x 150mm
| 87668
|
| Rocket?: 7 x 33mm
| 87671
|
| Rocket?: 7 x 53mm
| 87672
|
C18, 5μm
| Microbore: 2.1 x 150mm
| 87681
|
| Microbore: 2.1 x 250mm
| 87682
|
| Analytical: 4.6 x 150mm
| 87679
|
| Analytical: 4.6 x 250mm
| 87680
|
C18 AQ, 3μm
| Capillary: 0.150 x 150mm
| 22562
|
| Capillary: 0.300 x 150mm
| 22565
|
| Expedite? MS: 2.1 x 10mm
| 87813
|
| Expedite? MS: 2.1 x 20mm
| 87814
|
| Microbore: 2.1 x 150mm
| 87810
|
| Analytical: 4.6 x 50mm
| 87832
|
| Analytical: 4.6 x 150mm
| 87808
|
| Rocket?: 7 x 33mm
| 87811
|
| Rocket?: 7 x 53mm
| 87812
|
C18 AQ, 5μm
| Microbore: 2.1 x 150mm
| 87821
|
| Microbore: 2.1 x 250mm
| 87822
|
| Analytical: 4.6 x 150mm
| 87819
|
| Analytical: 4.6 x 250mm
| 87820
|
C18 EPS, 3μm
| Expedite? MS: 2.1 x 10mm
| 87709
|
| Expedite? MS: 2.1 x 20mm
| 87710
|
| Microbore: 2.1x 50mm
| 87508
|
| Microbore: 2.1 x 150mm
| 87706
|
| Analytical: 4.6 x 50mm
| 87833
|
| Analytical: 4.6 x 150mm
| 87704
|
| Rocket?: 7 x 33mm
| 87707
|
| Rocket?: 7 x 53mm
| 87708
|
C18 EPS, 5μm
| Microbore: 2.1 x 150mm
| 87717
|
| Microbore: 2.1 x 250mm
| 87718
|
| Analytical: 4.6 x 150mm
| 87715
|
| Analytical: 4.6 x 250mm
| 87716
|
C18 Hi-Load, 3μm
| Capillary: 0.150 x 50mm
| 22190
|
| Capillary: 0.300 x 50mm
| 22193
|
| Expedite? MS: 2.1 x 10mm
| 87691
|
| Expedite? MS: 2.1 x 20mm
| 87692
|
| Microbore: 2.1x 50mm
| 87506
|
| Microbore: 2.1 x 150mm
| 87688
|
| Analytical: 4.6 x 50mm
| 87827
|
| Analytical: 4.6 x 150mm
| 87686
|
| Rocket?: 7 x 33mm
| 87689
|
| Rocket?: 7 x 53mm
| 87690
|
C18 Hi-Load, 5μm
| Microbore: 2.1 x 150mm
| 87699
|
| Microbore: 2.1 x 250mm
| 87700
|
| Analytical: 4.6 x 150mm
| 87697
|
| Analytical: 4.6 x 250mm
| 87698
|
C18 Amide, 3μm
| Capillary: 0.150 x 50mm
| 22255
|
| Capillary: 0.300 x 50mm
| 22258
|
| Expedite? MS: 2.1 x 10mm
| 87727
|
| Expedite? MS: 2.1 x 20mm
| 87728
|
| Microbore: 2.1x 50mm
| 87510
|
| Microbore: 2.1 x 150mm
| 87724
|
| Analytical: 4.6 x 50mm
| 87829
|
| Analytical: 4.6 x 150mm
| 87722
|
| Rocket?: 7 x 33mm
| 87725
|
| Rocket?: 7 x 53mm
| 87726
|
C18 Amide, 5μm
| Microbore: 2.1 x 150mm
| 87735
|
| Microbore: 2.1 x 250mm
| 87736
|
| Analytical: 4.6 x 150mm
| 87733
|
| Analytical: 4.6 x 250mm
| 87734
|
C8, 3μm
| Capillary: 0.150 x 150mm
| 22481
|
| Capillary: 0.300 x 150mm
| 22484
|
| Expedite? MS: 2.1 x 10mm
| 87745
|
| Expedite? MS: 2.1 x 20mm
| 87746
|
| Microbore: 2.1x 50mm
| 87512
|
| Microbore: 2.1 x 150mm
| 87742
|
| Analytical: 4.6 x 50mm
| 87830
|
| Analytical: 4.6 x 150mm
| 87740
|
| Rocket?: 7 x 33mm
| 87743
|
| Rocket?: 7 x 53mm
| 87744
|
C8, 5μm
| Microbore: 2.1 x 150mm
| 87753
|
| Microbore: 2.1 x 250mm
| 87754
|
| Analytical: 4.6 x 150mm
| 87751
|
| Analytical: 4.6 x 250mm
| 87752
|
HILIC 1.5μm
| Expedite? MS: 2.1 x 20mm
| 86471
|
| Rocket?: 7 x 33mm
| 86467
|
HILIC 3μm
| Capillary: 0.150 x 150mm
| 22517
|
| Capillary: 0.300 x 150mm
| 22520
|
| Expedite? MS: 2.1 x 10mm
| 86473
|
| Expedite? MS: 2.1 x 20mm
| 86475
|
| Microbore: 2.1 x 50mm
| 86461
|
| Microbore: 2.1 x 150mm
| 86463
|
| Analytical: 4.6 x 50mm
| 86460
|
| Analytical: 4.6 x 150mm
| 86462
|
| Rocket?: 7 x 33mm
| 86469
|
| Rocket?: 7 x 53mm
| 86470
|
HILIC 5μm
| Microbore: 2.1 x 150mm
| 86465
|
| Analytical: 4.6 x 150mm
| 86464
|
| Analytical: 4.6 x 250mm
| 86466
|
Cyano 3μm
| Expedite? MS: 2.1 x 10mm
| 87763
|
| Expedite? MS: 2.1 x 20mm
| 87764
|
| Microbore: 2.1 x 50mm
| 87514
|
| Microbore: 2.1 x 150mm
| 87760
|
| Analytical: 4.6 x 50mm
| 87825
|
| Analytical: 4.6 x 150mm
| 87758
|
| Rocket?: 7 x 33mm
| 87761
|
| Rocket?: 7 x 53mm
| 87762
|
Cyano 5μm
| Microbore: 2.1 x 150mm
| 87781
|
| Microbore: 2.1 x 250mm
| 87782
|
| Analytical: 4.6 x 150mm
| 87769
|
| Analytical: 4.6 x 250mm
| 87780
|
Silica 3μm
| Expedite? MS: 2.1 x 10mm
| 87791
|
| Expedite? MS: 2.1 x 20mm
| 87792
|
| Microbore: 2.1 x 50mm
| 87516
|
| Microbore: 2.1 x 150mm
| 87788
|
| Analytical: 4.6 x 50mm
| 87831
|
| Analytical: 4.6 x 150mm
| 87786
|
| Rocket?: 7 x 33mm
| 87789
|
| Rocket?: 7 x 53mm
| 87790
|
Silica 5μm
| Microbore: 2.1 x 150mm
| 87799
|
| Microbore: 2.1 x 250mm
| 87802
|
| Analytical: 4.6 x 150mm
| 87797
|
| Analytical: 4.6 x 250mm
| 87798
|
用于保护毛细管柱的卡套与柱芯:
|
0.100mmID 毛细管柱保护卡套,1 套
| GR-3710E
|
0.300mmID 毛细管柱保护卡套,1 套
| GR-3710A
|
C18 毛细管保护柱芯 3μm
| 0.150 x 10mm
| 22578
|
0.300 x 10mm
| 22579
|
C18 AQ 毛细管保护柱芯 3μm
| 0.150 x 10mm
| 22638
|
0.300 x 10mm
| 22639
|
C18 Hi-Load 毛细管保护柱芯 3μm
| 0.150 x 10mm
| 22588
|
0.300 x 10mm
| 22589
|
C18 Amide 毛细管保护柱芯 3μm
| 0.150 x 10mm
| 22598
|
0.300 x 10mm
| 22599
|
C8 毛细管保护柱芯 3μm
| 0.150 x 10mm
| 22648
|
0.300 x 10mm
| 22649
|
HILIC 毛细管保护柱芯 3μm
| 0.150 x 10mm
| 22658
|
0.300 x 10mm
| 22659
|
用于保护分析柱的卡套与柱芯:
|
All-Guard保护柱卡套(含柱间连接件)1 套
| 80101
|
C18 保护柱芯 5μm
| 4.6 x 7.5mm, 3/pk
| 87684
|
2.1 x 7.5mm, 3/pk
| 87683
|
C18 AQ 保护柱芯 5μm
| 4.6 x 7.5mm, 3/pk
| 87824
|
2.1 x 7.5mm, 3/pk
| 87823
|
C18 EPS 保护柱芯 5μm
| 4.6 x 7.5mm, 3/pk
| 87720
|
2.1 x 7.5mm, 3/pk
| 87719
|
C18 Hi-Load 保护柱芯 5μm
| 4.6 x 7.5mm, 3/pk
| 87702
|
2.1 x 7.5mm, 3/pk
| 87701
|
C18 Amide 保护柱芯 5um
| 4.6 x 7.5mm, 3/pk
| 87738
|
2.1 x 7.5mm, 3/pk
| 87737
|
C8 保护柱芯5μm
| 4.6 x 7.5mm, 3/pk
| 87756
|
2.1 x 7.5mm, 3/pk
| 87755
|
HILIC 保护柱芯5μm
| 4.6 x 7.5mm, 3/pk
| 86480
|
2.1 x 7.5mm, 3/pk
| 86479
|
Cyano 保护柱芯 5μm
| 4.6 x 7.5mm, 3/pk
| 87784
|
2.1 x 7.5mm, 3/pk
| 87783
|
Silica 保护柱芯 5μm
| 4.6 x 7.5mm, 3/pk
| 87804
|
2.1 x 7.5mm, 3/pk
| 87803 |