大鼠速激肽受体2(TACR2)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠速激肽受体2(TACR2)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠速激肽受体2(TACR2)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠速激肽受体2(TACR2)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠速激肽受体2(TACR2)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-MK0669c 猴神经肽Y受体Y5(NPYR5)检测试剂盒 Monkey NPYR5 (Neuropeptide Y Receptor Y5) ELISA Kit
HZ-EL-MK0670c 猴Bcl-2样蛋白1(BCL2L1/BCL-X)检测试剂盒 Monkey BCL2L1/BCL-X (Bcl-2 Like Protein 1) ELISA Kit
HZ-EL-MK0671c 猴粘蛋白2(MUC2)检测试剂盒 Monkey MUC2 (Mucin 2) ELISA Kit
HZ-EL-MK0672c 猴内皮抑制素(ES)检测试剂盒 Monkey ES (Endostatin) ELISA Kit
HZ-EL-MK0673c 猴鸟氨酸氨基甲酰转移酶(OCT)检测试剂盒 Monkey OCT (Ornithine Carbamoyl Transferase) ELISA Kit
HZ-EL-MK0674c 猴5脂加氧酶(5-LO)检测试剂盒 Monkey 5-LO (Arachidonate 5-Lipoxygenase) ELISA Kit
HZ-EL-MK0675c 猴着丝粒蛋白E(CENPE)检测试剂盒 Monkey CENPE (Centromere Protein E) ELISA Kit
HZ-EL-MK0676c 猴激肽释放酶7(KLK7)检测试剂盒 Monkey KLK7 (Kallikrein 7) ELISA Kit
HZ-EL-MK0677c 猴肿瘤坏死因子β(TNF-β)检测试剂盒 Monkey TNF-β (Tumor Necrosis Factor Beta) ELISA Kit
HZ-EL-MK0678c 猴发动蛋白2(DNM2)检测试剂盒 Monkey DNM2 (Dynamin 2) ELISA Kit
HZ-EL-MK0679c 猴血管生成素(ANG)检测试剂盒 Monkey ANG (Angiogenin) ELISA Kit
HZ-EL-MK0680c 猴血小板激活因子(PAF)检测试剂盒 Monkey PAF (Platelet Activating Factor) ELISA Kit
HZ-EL-MK0681c 猴p53上调凋亡调节因子(PUMA)检测试剂盒 Monkey PUMA (P53 Upregulated Modulator of Apoptosis) ELISA Kit
HZ-EL-MK0682c 猴Sp100核抗原(Sp100)检测试剂盒 Monkey Sp100 (Sp100 Nuclear Antigen) ELISA Kit
HZ-EL-MK0683c 猴热休克蛋白90(HSP-90)检测试剂盒 Monkey HSP-90 (Heat Shock Protein 90) ELISA Kit
HZ-EL-MK0685c 猴嗜酸粒细胞趋化因子受体(ECFR)检测试剂盒 Monkey ECFR (Eosinophil Chemotactic Factor Receptor) ELISA Kit
HZ-EL-MK0686c 猴游离甲状腺素(fT4)检测试剂盒 Monkey fT4 (Free Thyroxine) ELISA Kit
HZ-EL-MK0687c 猴B细胞淋巴瘤因子10(Bcl-10)检测试剂盒 Monkey Bcl-10 (B-cell Leukemia/Lymphoma 10) ELISA Kit
HZ-EL-MK0688c 猴核因子κB受体激活因子(RANκ)检测试剂盒 Monkey RANκ (Receptor Activator of Nuclear Factor Kappa B) ELISA Kit
HZ-EL-MK0689c 猴腺苷酸环化酶4(ADCY4)检测试剂盒 Monkey ADCY4 (Adenylate Cyclase 4) ELISA Kit
HZ-EL-MK0690c 猴免疫球蛋白G Fc段受体II(FcγR II/CD32)检测试剂盒 Monkey FcγR II/CD32 (Receptor II for the Fc Fragment of IgG) ELISA Kit
HZ-EL-MK0691c 猴肽基精氨酸脱亚氨酶IV(PADI4)检测试剂盒 Monkey PADI4 (Peptidyl Arginine Deiminase Type IV) ELISA Kit
HZ-EL-MK0692c 猴芳香烃受体(AhR)检测试剂盒 Monkey AhR (Aryl Hydrocarbon Receptor) ELISA Kit
HZ-EL-MK0693c 猴粒细胞集落刺激因子(CSF3/GCSF)检测试剂盒 Monkey CSF3/GCSF (Granulocyte Colony Stimulating Factor 3) ELISA Kit
HZ-EL-MK0695c 猴细胞周期素D2(CCND2)检测试剂盒 Monkey CCND2 (Cyclin-D2) ELISA Kit