大鼠胆碱乙酰转移酶(ChAT)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠胆碱乙酰转移酶(ChAT)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠胆碱乙酰转移酶(ChAT)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠胆碱乙酰转移酶(ChAT)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠胆碱乙酰转移酶(ChAT)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-MK0612c 猴内凝集蛋白1(ITLN1)检测试剂盒 Monkey ITLN1 (Intelectin 1/Omentin) ELISA Kit
HZ-EL-MK0615c 猴皮肤T淋巴细胞相关抗原(CTAGE)检测试剂盒 Monkey CTAGE (Cutaneous T-Cell Lymphocyte Associated Antigen) ELISA Kit
HZ-EL-MK0616c 猴转录激活因子-5(ATF-5)检测试剂盒 Monkey ATF-5 (Activating Transcription Factor 5) ELISA Kit
HZ-EL-MK0617c 猴微粒体谷胱甘肽S转移酶1(MGST1)检测试剂盒 Monkey MGST1 (Microsomal Glutathione S Transferase 1) ELISA Kit
HZ-EL-MK0619c 猴基质金属蛋白酶25(MMP-25)检测试剂盒 Monkey MMP-25 (Matrix Metalloproteinase 25) ELISA Kit
HZ-EL-MK0620c 猴闭锁蛋白/遮光蛋白(OCLN)检测试剂盒 Monkey OCLN (Occludin) ELISA Kit
HZ-EL-MK0622c 猴肌腱蛋白C(TNC)检测试剂盒 Monkey TNC (Tenascin C) ELISA Kit
HZ-EL-MK0623c 猴原卟啉(EPP)检测试剂盒 Monkey EPP (Erythrocyte Protoporphyrin) ELISA Kit
HZ-EL-MK0624c 猴免疫球蛋白A1 (IgA1)检测试剂盒 Monkey IgA1 (Immunoglobulin A1) ELISA Kit
HZ-EL-MK0625c 猴肌肉生长抑制素(MSTN)检测试剂盒 Monkey MSTN (Myostatin) ELISA Kit
HZ-EL-MK0627c 猴表皮生长因子受体(EGFR)检测试剂盒 Monkey EGFR (Epidermal Growth Factor Receptor) ELISA Kit
HZ-EL-MK0628c 猴尿游离皮质醇(UFC)检测试剂盒 Monkey UFC (Urinary Free Cortisol) ELISA Kit
HZ-EL-MK0629c 猴血小板衍生生长因子C(PDGFC)检测试剂盒 Monkey PDGFC (Platelet Derived Growth Factor C) ELISA Kit
HZ-EL-MK0630c 猴G蛋白偶联受体120(GPR120)检测试剂盒 Monkey GPR120 (G Protein Coupled Receptor 120) ELISA Kit
HZ-EL-MK0631c 猴补体因子B(CFB)检测试剂盒 Monkey CFB (Complement Factor B) ELISA Kit
HZ-EL-MK0632c 猴XVII型胶原(COL17)检测试剂盒 Monkey COL17 (Collagen Type XVII) ELISA Kit
HZ-EL-MK0633c 猴组织蛋白酶B(CTSB)检测试剂盒 Monkey CTSB (Cathepsin B) ELISA Kit
HZ-EL-MK0634c 猴胰岛素样生长因子2-mRNA结合蛋白3(IGF2BP3)检测试剂盒 Monkey IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3) ELISA Kit
HZ-EL-MK0635c 猴重酒石酸去甲肾上腺素(NEB)检测试剂盒 Monkey NEB (Noradrenaline Bitartas) ELISA Kit
HZ-EL-MK0636c 猴Apelin蛋白(APLN)检测试剂盒 Monkey APLN (Apelin) ELISA Kit
HZ-EL-MK0637c 猴B细胞分化因子(BCDF)检测试剂盒 Monkey BCDF (B-Cell Differentiation Factor) ELISA Kit
HZ-EL-MK0638c 猴Bcl2关联永生基因3(BAG3)检测试剂盒 Monkey BAG3 (Bcl2 Associated Athanogene 3) ELISA Kit
HZ-EL-MK0640c 猴原钙黏素1(PCDH1)检测试剂盒 Monkey PCDH1 (Protocadherin 1) ELISA Kit
HZ-EL-MK0641c 猴谷氨酸脱羧酶(GAD)检测试剂盒 Monkey GAD (Glutamic Acid Decarboxylase) ELISA Kit
HZ-EL-MK0642c 猴间隙连接蛋白31(CX31)检测试剂盒 Monkey CX31 (Connexin 31) ELISA Kit
HZ-EL-MK0643c 猴细胞色素P450家族成员7A1(CYP7A1)检测试剂盒 Monkey CYP7A1 (Cytochrome P450 7A1) ELISA Kit