大鼠类固醇生成因子1(SF1)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠类固醇生成因子1(SF1)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠类固醇生成因子1(SF1)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠类固醇生成因子1(SF1)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠类固醇生成因子1(SF1)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-MK0237c 猴髓鞘碱性蛋白(MBP)检测试剂盒 Monkey MBP (Myelin Basic Protein) ELISA Kit
HZ-EL-MK0238c 猴丝氨酸蛋白酶23(PRSS23)检测试剂盒 Monkey PRSS23 (Protease, Serine 23) ELISA Kit
HZ-EL-MK0239c 猴骨成型蛋白10(BMP-10)检测试剂盒 Monkey BMP-10 (Bone Morphogenetic Protein 10) ELISA Kit
HZ-EL-MK0240c 猴蛋白磷酸酶(PP)检测试剂盒 Monkey PP (Protein Phosphatase) ELISA Kit
HZ-EL-MK0241c 猴核因子相关κB结合蛋白 (NFRκB)检测试剂盒 Monkey NFRκB (Nuclear Factor Related To Kappa B Binding Protein) ELISA Kit
HZ-EL-MK0242c 猴黏膜地址素细胞黏附分子(MAdCAM1)检测试剂盒 Monkey MAdCAM1 (Mucosal Addressin Cell Adhesion Molecule 1) ELISA Kit
HZ-EL-MK0243c 猴信号识别颗粒(SRP9)检测试剂盒 Monkey SRP9 (Signal Recognition Particle 9kDa) ELISA Kit
HZ-EL-MK0244c 猴泛素结合酶E2A(UBE2A)检测试剂盒 Monkey UBE2A (Ubiquitin Conjugating Enzyme E2A) ELISA Kit
HZ-EL-MK0245c 猴嗜酸粒细胞趋化因子(ECF/CCL11)检测试剂盒 Monkey ECF/CCL11 (Eosinophil Chemotactic Factor) ELISA Kit
HZ-EL-MK0246c 猴周期素依赖性激酶1(CDK1)检测试剂盒 Monkey CDK1 (Cyclin Dependent Kinase 1) ELISA Kit
HZ-EL-MK0247c 猴克拉拉细胞蛋白(CC16)检测试剂盒 Monkey CC16 (Clara Cell 16KD Protein) ELISA Kit
HZ-EL-MK0248c 猴血管紧张素I(ANG I)检测试剂盒 Monkey ANG I (Angiotensin I) ELISA Kit
HZ-EL-MK0249c 猴细胞外基质蛋白1(ECM1)检测试剂盒 Monkey ECM1 (Extracellular Matrix Protein 1) ELISA Kit
HZ-EL-MK0250c 猴皮肤T细胞虏获趋化因子(CTACK)检测试剂盒 Monkey CTACK (Cutaneous T-cell Attracting Chemokine) ELISA Kit
HZ-EL-MK0251c 猴胆囊收缩素/肠促胰酶肽A受体(CCKAR)检测试剂盒 Monkey CCKAR (Cholecystokinin A Receptor) ELISA Kit
HZ-EL-MK0252c 猴封闭蛋白9(CLDN9)检测试剂盒 Monkey CLDN9 (Claudin 9) ELISA Kit
HZ-EL-MK0253c 猴诱导型一氧化氮合酶(NOS2/iNOS)检测试剂盒 Monkey NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit
HZ-EL-MK0254c 猴穿孔蛋白1/成孔蛋白(PRF1/PFP)检测试剂盒 Monkey PRF1 (Perforin 1/Pore-Forming Protein) ELISA Kit
HZ-EL-MK0255c 猴长效甲状腺刺激素(LATS)检测试剂盒 Monkey LATS (Long Acting Thyroid Stimulator) ELISA Kit
HZ-EL-MK0256c 猴卵泡刺激素受体(FSHR)检测试剂盒 Monkey FSHR (Follicle Stimulating Hormone Receptor) ELISA Kit
HZ-EL-MK0257c 猴前列腺酸性磷酸酶(ACP3/PAP)检测试剂盒 Monkey ACP3/PAP (Prostatic Acid Phosphatase 3) ELISA Kit
HZ-EL-MK0258c 猴丙酮酸脱氢酶β(PDHβ)检测试剂盒 Monkey PDHβ (Pyruvate Dehydrogenase Beta) ELISA Kit
HZ-EL-MK0259c 猴新蝶呤 (Neopterin)检测试剂盒 Monkey Neopterin ELISA Kit
HZ-EL-MK0260c 猴可溶性血纤蛋白单体复合物(sFMC)检测试剂盒 Monkey sFMC (Soluble Fibrin Monomer Complex) ELISA Kit
HZ-EL-MK0261c 猴凋亡相关因子(FAS/CD95/TNFRSF6)检测试剂盒 Monkey FAS/CD95/TNFRSF6 (Factor Related Apoptosis) ELISA Kit
HZ-EL-MK0262c 猴乳酸脱氢酶C(LDHC)检测试剂盒 Monkey LDHC (Lactate Dehydrogenase C) ELISA Kit
HZ-EL-MK0264c 猴V-Myc骨髓细胞瘤病毒癌基因同源物(MYC)检测试剂盒 Monkey MYC (V-Myc Myelocytomatosis Viral Oncogene Homolog) ELISA Kit