大鼠生长抑素(SST)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠生长抑素(SST)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠生长抑素(SST)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠生长抑素(SST)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠生长抑素(SST)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-MK0122c 猴乳酸脱氢酶(LDH)检测试剂盒 Monkey LDH (Lactate Dehydrogenase) ELISA Kit
HZ-EL-MK0123c 猴间隙连接蛋白37(CX37)检测试剂盒 Monkey CX37 (Connexin 37) ELISA Kit
HZ-EL-MK0124c 猴CCAAT增强子结合蛋白ε(C/EBPε)检测试剂盒 Monkey C/EBPε (CCAAT/Enhancer Binding Protein, Epsilon) ELISA Kit
HZ-EL-MK0125c 猴丝裂原激活蛋白激酶激酶6(MAP2K6/MKK6)检测试剂盒 Monkey MAP2K6/MKK6 (Mitogen Activated Protein Kinase Kinase 6) ELISA Kit
HZ-EL-MK0127c 猴丙酮酸脱氢酶激酶同工酶4(PDK4)检测试剂盒 Monkey PDK4 (Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA Kit
HZ-EL-MK0128c 猴γ谷氨酰转移酶1(γGT1)检测试剂盒 Monkey γGT1 (Gamma Glutamyltransferase 1) ELISA Kit
HZ-EL-MK0129c 猴别孕烯醇酮 (AP)检测试剂盒 Monkey AP (Allopregnanolone) ELISA Kit
HZ-EL-MK0130c 猴细胞色素b-561(CYB561)检测试剂盒 Monkey CYB561 (Cytochrome b-561) ELISA Kit
HZ-EL-MK0131c 猴结构特异性识别蛋白1(SSRP1)检测试剂盒 Monkey SSRP1 (Structure Specific Recognition Protein 1) ELISA Kit
HZ-EL-MK0132c 猴富含半胱氨酸蛋白61(Cyr61)检测试剂盒 Monkey Cyr61 (Cysteine Rich Protein, Angiogenic Inducer 61) ELISA Kit
HZ-EL-MK0133c 猴转录激活因子6(ATF6)检测试剂盒 Monkey ATF6 (Activating Transcription Factor 6) ELISA Kit
HZ-EL-MK0134c 猴甲状腺激素反应基因(THRSP)检测试剂盒 Monkey THRSP (Thyroid Hormone Responsive) ELISA Kit
HZ-EL-MK0135c 猴溶菌酶(LZM)检测试剂盒 Monkey LZM (Lysozyme) ELISA Kit
HZ-EL-MK0136c 猴血管内皮细胞生长因子受体1(VEGFR1/Flt1)检测试剂盒 Monkey VEGFR1/Flt1 (Vascuoar Endothelial Growth Factor Receptor 1) ELISA Kit
HZ-EL-MK0137c 猴抗髓磷脂抗体IgA(AMA IgA)检测试剂盒 Monkey AMA IgA (Anti-Myelin antibody IgA) ELISA Kit
HZ-EL-MK0138c 猴衰老关键蛋白5(FBLN5)检测试剂盒 Monkey FBLN5 (Fibulin 5) ELISA Kit
HZ-EL-MK0139c 猴食欲素/阿立新B(OXB)检测试剂盒 Monkey OXB (Orexin B) ELISA Kit
HZ-EL-MK0140c 猴抗补体1q抗体(C1q-Ab)检测试剂盒 Monkey C1q-Ab (Anti-Complement 1q Antibody) ELISA Kit
HZ-EL-MK0141c 猴睫状神经营养因子(CNTF)检测试剂盒 Monkey CNTF (Ciliary Neurotrophic Factor) ELISA Kit
HZ-EL-MK0142c 猴白介素22(IL-22)检测试剂盒 Monkey IL-22 (Interleukin 22) ELISA Kit
HZ-EL-MK0143c 猴碱性唾液脯氨酸丰富蛋白2(PRB2)检测试剂盒 Monkey PRB2 (Basic Salivary Proline Rich Protein 2) ELISA Kit
HZ-EL-MK0144c 猴角膜锁链蛋白(CDSN)检测试剂盒 Monkey CDSN (Corneodesmosin) ELISA Kit
HZ-EL-MK0146c 猴肌腱蛋白R(TNR)检测试剂盒 Monkey TNR (Tenascin R) ELISA Kit
HZ-EL-MK0147c 猴凝集素样氧化低密度脂蛋白受体1(LOX-1)检测试剂盒 Monkey LOX-1 (Lectin Like Oxidized Low Density Lipoprotein Receptor 1) ELISA Kit
HZ-EL-MK0148c 猴S100蛋白(S-100)检测试剂盒 Monkey S-100 (Soluble Protein-100) ELISA Kit