大鼠过氧化物酶体膜蛋白3(PMP 3)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠过氧化物酶体膜蛋白3(PMP 3)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠过氧化物酶体膜蛋白3(PMP 3)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠过氧化物酶体膜蛋白3(PMP 3)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠过氧化物酶体膜蛋白3(PMP 3)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-RB1206c 兔α-内吗啡肽(α-EP)检测试剂盒 Rabbit α-EP (α-Endomorphin) ELISA Kit
HZ-EL-RB1207c 兔谷氧还蛋白3(GLRX3)检测试剂盒 Rabbit GLRX3 (Glutaredoxin 3) ELISA Kit
HZ-EL-RB1208c 兔免疫球蛋白A (IgA)检测试剂盒 Rabbit IgA (Immumoglobulin A) ELISA Kit
HZ-EL-RB1209c 兔STAT蛋白抑制因子3(PIAS3)检测试剂盒 Rabbit PIAS3 (Protein Inhibitor of Activated STAT3) ELISA Kit
HZ-EL-RB1210c 兔促红细胞生成素(EPO)检测试剂盒 Rabbit EPO (Erythropoietin) ELISA Kit
HZ-EL-RB1211c 兔脂多糖结合蛋白(LBP)检测试剂盒 Rabbit LBP (Lipopolysaccharide Binding Protein) ELISA Kit
HZ-EL-RB1212c 兔甲状腺素受体α(THRα)检测试剂盒 Rabbit THRα (Thyroid Hormone Receptor Alpha) ELISA Kit
HZ-EL-RB1213c 兔CCAAT增强子结合蛋白β(C/EBPβ)检测试剂盒 Rabbit C/EBPβ (CCAAT/Enhancer Binding Protein Beta) ELISA Kit
HZ-EL-RB1214c 兔5脂加氧酶(5-LO)检测试剂盒 Rabbit 5-LO (Arachidonate 5-Lipoxygenase) ELISA Kit
HZ-EL-RB1215c 兔蛋白聚糖(PG)检测试剂盒 Rabbit PG (Proteoglycan) ELISA Kit
HZ-EL-RB1216c 兔细胞绒毛蛋白(CVL/EZR)检测试剂盒 Rabbit CVL/EZR (Cytovillin/Ezrin) ELISA Kit
HZ-EL-RB1217c 兔白介素2(IL-2)检测试剂盒 Rabbit IL-2 (Interleukin 2) ELISA Kit
HZ-EL-RB1218c 兔甘露糖凝集素受体(MBLR)检测试剂盒 Rabbit MBLR (Mannose Binding Lectin Receptor) ELISA Kit
HZ-EL-RB1219c 兔周期素依赖性激酶1(CDK1)检测试剂盒 Rabbit CDK1 (Cyclin Dependent Kinase 1) ELISA Kit
HZ-EL-RB1220c 兔TU3A蛋白(TU3A)检测试剂盒 Rabbit TU3A (TU3A Protein) ELISA Kit
HZ-EL-RB1221c 兔半胱氨酰白三烯受体1(CYSLTR1)检测试剂盒 Rabbit CYSLTR1 (Cysteinyl Leukotriene Receptor 1) ELISA Kit
HZ-EL-RB1222c 兔促性腺激素释放激素(GnRH)检测试剂盒 Rabbit GnRH (Gonadotropin Releasing Hormone) ELISA Kit
HZ-EL-RB1223c 兔催乳素(PRL)检测试剂盒 Rabbit PRL (Prolactin) ELISA Kit
HZ-EL-RB1225c 兔补体成分5a(C5a)检测试剂盒 Rabbit C5a (Complement Component 5a) ELISA Kit
HZ-EL-RB1226c 兔淋巴细胞趋化因子(Lptn/XCL1)检测试剂盒 Rabbit Lptn/XCL1 (Lymphotactin) ELISA Kit
HZ-EL-RB1227c 兔Ⅱ型胶原螺旋肽(HELIX-Ⅱ)检测试剂盒 Rabbit HELIX-Ⅱ(Type Ⅱ Collagen Helical Peptide) ELISA Kit
HZ-EL-RB1228c 兔视黄酸诱导基因/RIG-1样受体(RLR)检测试剂盒 Rabbit RLR (Retinoic Acid Inducible Gene/RIG-Like Receptor) ELISA Kit
HZ-EL-RB1229c 兔卷曲同源物8(FZD8)检测试剂盒 Rabbit FZD8 (Frizzled Homolog 8) ELISA Kit
HZ-EL-RB1230c 兔胱天蛋白酶12(Casp12)检测试剂盒 Rabbit CASP12 (Caspase 12) ELISA Kit
HZ-EL-RB1231c 兔促甲状腺激素抗体(TSA)检测试剂盒 Rabbit TSA (Thyroid Stimulating Antibody) ELISA Kit
HZ-EL-RB1232c 兔血管生成素样蛋白1(ANGPTL1)检测试剂盒 Rabbit ANGPTL1 (Angiopoietin Like Protein 1) ELISA Kit
HZ-EL-RB1233c 兔间隙连接蛋白40(CX40)检测试剂盒 Rabbit CX40 (Connexin 40) ELISA Kit