小鼠胆固醇 (CH) 检测试剂盒说明上写封闭液用蔗糖溶液,一般是用BSA或酪蛋白的蛋白分子封闭空白位点,蔗糖封闭的原理:
1、小鼠胆固醇 (CH) 检测试剂盒实验中,蔗糖本身没有封闭作用;
2、封闭液一般是BSA、酪蛋白等或者是Tween等。
3、加蔗糖的主要目的是保护ELISA板子吸附(包被)的蛋白,起到“保护剂”的作用。
4、蔗糖溶液一般在ELISA板子经过BSA封闭后加,当然也有将蔗糖加到封闭液中同时进行处理;那一种更好需要你实验后来验证。但多数选择前者。
封闭剂还可用5%的脱脂乳(市售即可),0.4%的明胶,但是实验表明前者比较好,后者不容易洗干净未结合上的明胶。
小鼠胆固醇 (CH) 检测试剂盒封闭剂的原理就是与酶联板上未结合抗原或抗体的微孔中用封闭剂封闭上,结合抗体或抗原的时候就不会产生非特异结合了,不会影响实验的准确度。
tween的作用:虽然不能单独封闭,但和BSA在封闭时会起到清洗非特异性结合或结合不牢固的蛋白的作用,假如抗体包被在板上会出现YY-
附件:小鼠胆固醇 (CH) 检测试剂盒实验中缓冲溶液等试剂的配置
ELISA相关产品,详情见:封闭试剂、终止溶液、封闭肽、ELISA稀释液、ELISA试剂盒、ELISA缓冲液、ELISA洗脱缓冲液
为了保证小鼠胆固醇 (CH) 检测试剂盒实验的准确性,实验操作中加入的缓冲液和各组分一定要精确,将洗涤液加入酶标板中的时候防止加入的试剂混入另外一个酶标孔,严防交叉污染。
HZ-EL-MK0701c 猴抗磷脂酰丝氨酸抗体(APSA)检测试剂盒 Monkey APSA (Anti-Phosphatidyl Serine Antibody) ELISA Kit
HZ-EL-MK0702c 猴补体片段3c(C3c)检测试剂盒 Monkey C3c (Complement Fragment 3c) ELISA Kit
HZ-EL-MK0703c 猴蛋白聚糖4(PRG4)检测试剂盒 Monkey PRG4 (Proteoglycan 4) ELISA Kit
HZ-EL-MK0704c 猴补体因子4 (C4)检测试剂盒 Monkey C4 (Complement Component 4) ELISA Kit
HZ-EL-MK0705c 猴骨形成蛋白6(BMP-6)检测试剂盒 Monkey BMP-6 (Bone Morphogenetic Protein 6) ELISA Kit
HZ-EL-MK0706c 猴谷胱甘肽S转移酶α4(GSTα4)检测试剂盒 Monkey GSTα4 (Glutathione S Transferase Alpha 4) ELISA Kit
HZ-EL-MK0707c 猴丝裂原激活蛋白激酶激活蛋白激酶3 (MAPKAPK3)检测试剂盒 Monkey MAPKAPK3 (Mitogen Activated Protein Kinase Activated Protein Kinase 3) ELISA Kit
HZ-EL-MK0708c 猴生长分化因子10(GDF10)检测试剂盒 Monkey GDF10 (Growth Differentiation Factor 10) ELISA Kit
HZ-EL-MK0709c 猴半乳凝集素1(GAL1)检测试剂盒 Monkey GAL1 (Galectin 1 ) ELISA Kit
HZ-EL-MK0710c 猴心肌营养素1(CT-1)检测试剂盒 Monkey CT-1 (Cardiotrophin 1) ELISA Kit
HZ-EL-MK0711c 猴淋巴细胞功能相关抗原1(LFA-1)检测试剂盒 Monkey LFA-1 (Lymphocyte Function Associated Antigen 1) ELISA Kit
HZ-EL-MK0712c 猴转录激活因子-1(ATF-1)检测试剂盒 Monkey ATF-1 (Activating Transcription Factor 1) ELISA Kit
HZ-EL-MK0713c 猴唾液酸结合免疫球蛋白样凝集素3(SIGLEC3)检测试剂盒 Monkey SIGLEC3 (Sialic Acid Binding Ig Like Lectin 3) ELISA Kit
HZ-EL-MK0714c 猴促红细胞生成素(EPO)检测试剂盒 Monkey EPO (Erythropoietin) ELISA Kit
HZ-EL-MK0715c 猴L选择素(SELL)检测试剂盒 Monkey SELL (L-Selectin) ELISA Kit
HZ-EL-MK0717c 猴血清淀粉样蛋白P(SAP)检测试剂盒 Monkey SAP (Serum Amyloid P Component) ELISA Kit
HZ-EL-MK0719c 猴基质细胞衍生因子2样蛋白1(SDF2L1)检测试剂盒 Monkey SDF2L1 (Stromal Cell Derived Factor 2 Like Protein 1) ELISA Kit
HZ-EL-MK0720c 猴C4结合蛋白(C4BP)检测试剂盒 Monkey C4BP (C4 Binding Protein) ELISA Kit
HZ-EL-MK0721c 猴胱天蛋白酶10(CASP10)检测试剂盒 Monkey CASP10 (Caspase 10) ELISA Kit
HZ-EL-MK0722c 猴信号传导转录激活因子3(STAT3)检测试剂盒 Monkey STAT3 (Signal Transducer and Activator of Transcription 3) ELISA Kit
HZ-EL-MK0723c 猴血红蛋白μ (HBμ)检测试剂盒 Monkey HBμ (Hemoglobin, Mu) ELISA Kit
HZ-EL-MK0725c 猴接触蛋白相关蛋白1(CNTNAP1)检测试剂盒 Monkey CNTNAP1 (Contactin Associated Protein 1) ELISA Kit
HZ-EL-MK0726c 猴轴突生长诱向因子1(Ntn1)检测试剂盒 Monkey Ntn1 (Netrin 1) ELISA Kit
HZ-EL-MK0727c 猴肝细胞生长因子(HGF)检测试剂盒 Monkey HGF (Hepatocyte Growth Factor) ELISA Kit