小鼠甲状旁腺素受体2(PTHR2)检测试剂盒免疫标记技术是将一些既易测定又具有高度敏感性的物质标记到特异性抗原或抗体分子上,通过这些标记物的增强放大效应来显示反应系统中抗原或抗体的性质与含量。常用的标记物包括荧光素、酶和放射性核素等,用这3种标记物进行标记的免疫检测技术被称为3大免疫标记技术。目前,使用的免疫标记物还有化学发光物质、铁蛋白和胶体金等。
小鼠甲状旁腺素受体2(PTHR2)检测试剂盒酶制剂及其底物
凡无毒性又能呈现有色化学反应的酶,原则上均可作为标记用。但作为标记抗体用的酶应满足下列要求:
(1)来源方便,易于纯化;
(2)比活性高,性质稳定;
(3)酶活性和量能
小鼠甲状旁腺素受体2(PTHR2)检测试剂盒用简单方法测定。目前在免疫酶技术中常用的酶为辣根过氧化物酶(HRP)和硷性磷酸酶(AP),其次还有葡萄糖氧化酶,β-半乳糖苷酶、溶菌酶和苹果酸脱氢酶等。
小鼠甲状旁腺素受体2(PTHR2)检测试剂盒由于辣根过氧化物酶(Horseradish Peroxidase, HRP)比活性高,稳定,分子量小,纯酶容易制备,所以最常用。HRP广泛分布于植物界,辣根中含量高,它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白,糖含量18%。HRP由多个同功酶组成,分子量为40,000,等电点为PH3~9,酶催化的最适PH因供氢体不同而稍有差异,但多在PH5左右。酶溶于水和58%以下饱和度硫酸铵溶液。HRP的辅基和酶蛋白最大吸收光谱分别为403nm和275nm,一般以OD403nm /OD275nm的比值RZ(德文Reinheit Zahl)表示酶的纯度。高纯度的酶RZ值应在3.0左右(最高可达3.4)。小鼠甲状旁腺素受体2(PTHR2)检测试剂盒RZ值越小,非酶蛋白就越多。值得注意的是,纯度并不表示酶活性,如当酶变性后,RZ值仍可不变。
HZ-EL-Ch0185c 鸡促睡眠肽α(δSIP-α)检测试剂盒 Chicken δSIP-α (Delta Sleep Inducing Peptide α) ELISA Kit
HZ-EL-Ch0186c 鸡神经纤维网蛋白1(NRP1)检测试剂盒 Chicken NRP1 (Neuropilin 1) ELISA Kit
HZ-EL-Ch0187c 鸡信号传导转录激活因子5A(STAT5A)检测试剂盒 Chicken STAT5A (Signal Transducer and Activator of Transcription 5A) ELISA Kit
HZ-EL-Ch0188c 鸡B细胞分化因子(BCDF)检测试剂盒 Chicken BCDF (B-Cell Differentiation Factor) ELISA Kit
HZ-EL-Ch0189c 鸡甘露聚糖结合凝集素相关丝氨酸蛋白酶(MASP)检测试剂盒 Chicken MASP (Mannan Binding Lectin Associated Serine Protease) ELISA Kit
HZ-EL-Ch0190c 鸡低氧上调节因子1(HYOU1)检测试剂盒 Chicken HYOU1 (Hypoxia Up Regulated 1) ELISA Kit
HZ-EL-Ch0191c 鸡黑素瘤衍生亮氨酸拉链额外核因子(MLZE)检测试剂盒 Chicken MLZE (Melanoma Derived Leucine Zipper Extra Nuclear Factor) ELISA Kit
HZ-EL-Ch0192c 鸡血小板衍生生长因子(PDGF)检测试剂盒 Chicken PDGF (Platelet-Derived Growth Factor) ELISA Kit
HZ-EL-Ch0193c 鸡钙调磷酸酶(CaN)检测试剂盒 Chicken CaN (Calcineurin) ELISA Kit
HZ-EL-Ch0194c 鸡细胞色素P450氧化还原酶(CPR)检测试剂盒 Chicken CPR (Cytochrome P450 Reductase) ELISA Kit
HZ-EL-Ch0196c 鸡周期素依赖性激酶抑制因子3(CDKN3)检测试剂盒 Chicken CDKN3 (Cyclin Dependent Kinase Inhibitor 3) ELISA Kit
HZ-EL-Ch0197c 鸡CXC趋化因子受体1(CXCR1)检测试剂盒 Chicken CXCR1 (CXC-Chemokine Receptor 1) ELISA Kit
HZ-EL-Ch0198c 鸡轴突生长诱向因子1(Ntn1)检测试剂盒 Chicken Ntn1 (Netrin 1) ELISA Kit
HZ-EL-Ch0200c 鸡抗胰岛素受体抗体(AIRA)检测试剂盒 Chicken AIRA (Anti-Insulin Receptor Antibody) ELISA Kit
HZ-EL-Ch0201c 鸡γ谷氨酰转移酶1(γGT1)检测试剂盒 Chicken γGT1 (Gamma Glutamyltransferase 1) ELISA Kit
HZ-EL-Ch0202c 鸡半乳凝集素12(GAL12)检测试剂盒 Chicken GAL12 (Galectin 12 ) ELISA Kit
HZ-EL-Ch0203c 鸡BCL2修饰因子(BMF)检测试剂盒 Chicken BMF (Bcl-2 Modifying Factor) ELISA Kit
HZ-EL-Ch0204c 鸡神经胶质成熟因子β(GMFβ)检测试剂盒 Chicken GMFβ (Glia Maturation Factor, Beta) ELISA Kit
HZ-EL-Ch0205c 鸡肝配蛋白A受体3 (EPHA3)检测试剂盒 Chicken EPHA3 (Ephrin Type A Receptor 3) ELISA Kit
HZ-EL-Ch0206c 鸡α-骨胶原交联(α-CTx)检测试剂盒 Chicken α-CTx (Alpha Crosslaps) ELISA Kit
HZ-EL-Ch0207c 鸡细胞周期素D3(CCND3)检测试剂盒 Chicken CCND3 (Cyclin-D3) ELISA Kit
HZ-EL-Ch0208c 鸡皮肤桥蛋白(DPT)检测试剂盒 Chicken DPT (Dermatopontin) ELISA Kit
HZ-EL-Ch0209c 鸡解整合素金属蛋白酶8(ADAM8)检测试剂盒 Chicken ADAM8 (A Disintegrin And Metalloprotease 8) ELISA Kit
HZ-EL-Ch0210c 鸡神经丝蛋白3(NEF3)检测试剂盒 Chicken NEF3 (Neurofilament 3) ELISA Kit
HZ-EL-Ch0211c 鸡基膜聚糖(LUM)检测试剂盒 Chicken LUM (Lumican) ELISA Kit
HZ-EL-Ch0212c 鸡高尔基蛋白73(GP-73)检测试剂盒 Chicken GP-73 (Golgi Protein 73) ELISA Kit
HZ-EL-Ch0213c 鸡集蛋白亚家族成员11(COLEC11)检测试剂盒 Chicken COLEC11 (Collectin Sub-Family Member 11) ELISA Kit
HZ-EL-Ch0214c 鸡吡哆醛激酶(PDXK)检测试剂盒 Chicken PDXK (Pyridoxal Kinase) ELISA Kit
HZ-EL-Ch0215c 鸡肿瘤坏死因子α(TNF-α)检测试剂盒 Chicken TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit
HZ-EL-Ch0216c 鸡谷胱甘肽S转移酶ω1(GSTω1)检测试剂盒 Chicken GSTω1 (Glutathione S Transferase Omega 1) ELISA Kit