超高速视频级原子力显微镜

日本RIBM  HS-AFM

超高速视频原子力显微镜

技术特征：

技术原理：

超高速视频原子力显微镜应用领域：

应用包括：利用 HS-AFM可在纳米尺度动态实时记录生物大分子的运动以及分子间相互作用，包括：     

IgG antibody 150nm * 150nm	plasmid DNA 250nm * 250nm	myosinⅡ 500nm * 500nm	bacteriorhodopsin 40nm * 40nm
lipid membrane 3500nm * 3500nm	350nm poly beads 900nm * 900nm	E.coli 3000nm * 3000nm	350nm poly beads 3000nm * 3000nm

超高速视频原子力显微镜相关应用案例：

 1.Video imaging of walking myosin V   实时观察myosin V蛋白的运动 

N. Kodera et al. Nature 468, 72 (2010). Kanazawa University  

2.Real-space and real-time dynamics of CRISPR-Cas9   实时显示CRISPR基因编辑

 Mikihiro et al. Nature Communications,  (2017). Kanazawa University

设备规格及参数：    

已发表文献（2017年）：

1. Ando T.; "Directly watching biomolecules in action by high-speed atomic force microscopy"; Biophys. Rev. (2017)

2. Ando T.; "High-speed Atomic Force Microscopy for Observing Protein Molecules in Dynamic Action", Proceedings of SPIE 10328, Selected Papers from the 31st International Congress on High-Speed Imaging and Photonics (2017)

3. Aybeke E., Belliot G., Lemaire‐Ewing S., Estienney M., Lacroute Y., Pothier P., Bourillot E., Lesniewska, E.; "HS‐AFM and SERS Analysis of Murine Norovirus Infection: Involvement of the Lipid Rafts"; Small 13 1 (2017)

4. Cai W, Liu Z., Chen Y., Shang G.; "A Mini Review of the Key Components used for the Development of High-Speed Atomic Force Microscopy"; Science of Advanced Materials Vol. 9 Numb. 1 (2017) p.77-88

5. Colom A., Redondo-Morata L., Chiaruttini N., Roux A., Scheuring S.; "Dynamic remodeling of the dynamin helix during membrane constriction"; Proceedings of the National Academy of Sciences 114 21 (2017)

6. Dufrêne Y., Ando T., Garcia R., Alsteens D., Martinez-Martin D., Engel A., Gerber Ch., Müller D.; "Imaging modes of atomic force microscopy for application of molecular and cell biology"; Nat. Nanotechnol. 12 (2017) p.295-307

7. Harada H., Onoda A., Uchihashi T., Watanabe H., Sunagawa N., Samejima M., Igarashi K., Hayashi T.; "Interdomain flip-flop motion visualized in flavocytochrome cellobiose dehydrogenase using high-speed atomic force microscopy during catalysis"; Chemical Science (2017)

8. Karner A., Nimmervoll B., Plochberger B., Klotzsch E., Horner A., Knyazev D., Kuttner R., Winkler K., Winter L., Siligan Ch., Ollinger N., Pohl P., Preiner J.; "Tuning membrane protein mobility by confinement into nanodomains"; Nature Nanotechnology 12 3 (2017) p.260-266

9. Keya J., Inoue D., Suzuki Y., Kozai T., Ishikuro D., Kodera N., Uchihashi T., Kabir A., Endo M., Sada K., Kakugo A.; "High-Resolution Imaging of a Single Gliding Protofilament of Tubulins by HS-AFM" ; Scientific Reports 7 1 (2017)

10. Kim Y.; "An Advanced Characterization Method for the Elastic Modulus of Nanoscale Thin-Films Using a High-Frequency Micromechanical Resonator"; Materials 10 7 (2017)

11. Kim Y.; "An evaluation technique for high-frequency dynamic behavior of a sandwich microcantilever beam"; Journal of Sandwich Structures & Materials (2017)

12. Korolkov V., Baldoni M., Watanabe K., Taniguchi T., Besley E., Beton P.; "Supramolecular heterostructures formed by sequential epitaxial deposition of two-dimensional hydrogen-bonded arrays"; Nature Chemistry (2017)

13. Legrand B., Salvetat J.-P., Walter B., Faucher M., Théron D., Aimé J.-P.; "Multi-MHz micro-electro-mechanical sensors for atomic force microscopy"; Ultramicroscopy 175 (2017) p.46-57

14. Liao H.-S., Chih-Wen Yang, Hsien-Chen Ko, En-Te Hwu, Ing-Shouh Hwang; "Imaging initial formation processes of nanobubbles at the graphite–water interface through high-speed atomic force microscopy"; Applied Surface Science (2017)

15. Matsui S., Kureha T., Hiroshige S., Shibata M., Uchihashi T., Suzuki D.; "Fast Adsorption of Soft Hydrogel Microspheres on Solid Surfaces in Aqueous Solution"; Angewandte Chemie (2017)

16. Mierzwa B., Chiaruttini N., Redondo-Morata L., Moser von Filseck J., K?nig J., Larios J., Poser I., Müller-Reichert T., Scheuring S., Roux A., Gerlich D.; "Dynamic subunit turnover in ESCRT-III assemblies is regulated by Vps4 to mediate membrane remodeling during cytokinesis"; Nature Cell Biology (2017)

17. Miyata K., Tracey J., Miyazawa K., Haapasilta V., Spijker P., Kawagoe Y., Foster A., Tsukamoto K., Fukuma T.; "Dissolution Processes at Step Edges of Calcite in Water Investigated by High-Speed Frequency Modulation Atomic Force Microscopy and Simulation"; Nano Lett. 17 7 (2017) p.4083-4089

18. Miyazawa K., Watkins M., Shluger A., Fukuma T.; "Influence of ions on two-dimensional and three-dimensional atomic force microscopy at fluorite–water interfaces"; Nanotechnology Vol. 28 Numb. 24 (2017)

19. Mohamed M., Kobayashi A., Taoka A., Watanabe-Nakayama T., Kikuchi Y., Hazawa M., Minamoto T., Fukumori Y., Kodera N., Uchihashi T., Ando T., Wong R.; "High-Speed Atomic Force Microscopy Reveals Loss of Nuclear Pore Resilience as a Dying Code in Colorectal Cancer Cells"; ACS Nano 11 6 (2017) p.5567-5578

20. Nievergelt A., Andany S., Adams J., Hannebelle M., Fantner G.; "Components for high-speed atomic force microscopy optimized for low phase-lag"; Proceedings of 2017 IEEE International Conference on Advanced Intelligent Mechatronics (AIM) (2017)

21. Rangl M., Rima L., Klement J., Miyagi A., Keller S., Scheuring S.; "Real-time Visualization of Phospholipid Degradation by Outer Membrane Phospholipase A using High-Speed Atomic Force Microscopy"; Journal of Molecular Biology 429 7 (2017) p.977-986

22. Ren J., Zou Q.; "High-speed dynamic-mode atomic force microscopy imaging of polymers: an adaptive multiloop-mode approach"; Beilstein J. Nanotechnol. 8 (2017) p.1563-1570

23. Ricci M., Trewby W., Cafolla C., Vo?tchovsky K.; "Direct observation of the dynamics of single metal ions at the interface with solids in aqueous solutions"; Scientific Reports 7 43234 (2017)

24. Rigato A., Miyagi A., Scheuring S., Rico F.; "High-frequency microrheology reveals cytoskeleton dynamics in living cells"; Nature Physics (2017) DOI: 10.1038/NPHYS4104

25. Ruan Y., Miyagi A., Wang X., Chami M., Boudker O., Scheuring S.; "Direct visualization of glutamate transporter elevator mechanism by high-speed AFM"; PNAS 114 7 (2017) p.1584-1588

26. Sadeghian H., Herfst R., Dekker B., Winters J., Bijnagte T., Rijnbeek R.; "High-throughput atomic force microscopes operating in parallel"; Review of Scientific Instruments 88 033703 (2017)

27. Sakiyama Y., Panatala R., Lim R.; "Structural Dynamics of the Nuclear Pore Complex"; Seminars in Cell and Developmental Biology (2017)

28. Shibata M., Watanabe H., Uchihashi T., Ando T., Yasuda R.; "High-speed atomic force microscopy imaging of live mammalian cells"; Biophysics and Physicobiology Vol. 14 (2017) p.127-135

29. Terahara N., Kodera N., Uchihashi T., Ando T., Namba K., Minamino T.; "Na+-induced structural transition of MotPS for stator assembly of the Bacillus flagellar motor"; Science Advances 3 11 eaao4119 (2017)

30. Uchihashi T., Scheuring S.; "Applications of high-speed atomic force microscopy to real-time visualization of dynamic biomolecular processes"; Biochim Biophys Acta. (2017)

31. Usukura E., Narita A., Yagi A., Sakai N., Uekusa Y., Imaoka Y., Ito S., Usukura J.; "A Cryosectioning Technique for the Observation of Intracellular Structures and Immunocytochemistry of Tissues in Atomic Force Microscopy (AFM)"; Scientific Reports 7 (2017) 

32. Watanabe S., Ando T.; "High-speed XYZ-nanopositioner for scanning ion conductance microscopy"; Applied Physics Letters 111 11 (2017)

33. Watanabe-Nakayama T., Kodera N., Konno H., Ono K., Teplow D., Yamada M., Ando T.; "Nano-Space Video Imaging Reveals Structural Dynamics of Fibrous Protein Assembly and Relevant Enzymes"; Biophysical Journal 112 3 (2017)

34. Zhang Y., Tunuguntla R., Choi P., Noy A.; "Real-time dynamics of carbon nanotube porins in supported lipid membranes visualized by high-speed atomic force microscopy"; Philosophical Transactions of The Royal Society B Biological Sciences 372 (2017)

35. Zhang Y., Yoshida A., Sakai N., Uekusa Y., Kumeta M., Yoshimura S.; "In vivo dynamics of the cortical actin network revealed by fast-scanning atomic force microscopy" Microscopy 20 (2017) p.272-282 

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