简介:Mouse Runx2
FOR RESEARCH USE ONLY
Assay range:2ng/L -85ng/L 96 determinations
Purpose
This kit allows for the determination of Runx2 concentrations in Mouse Hippocampal tissue and other biological fl简介:Mouse Runx2
FOR RESEARCH USE ONLY
Assay range:2ng/L -85ng/L 96 determinations
Purpose
This kit allows for the determination of Runx2 concentrations in Mouse Hippocampal tissue and other biological fluids.
Principle of the assay
The kit assay Mouse Runx2 level in the sample, use Purified Mouse Runx2 antibody to coat microtiter plate wells, make solid-phase antibody, then add Runx2 to wells, Combined Runx2 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Mouse Runx2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.详细>