简介:Rat Low density lipoprotein(LDL)
FOR RESEARCH USE ONLY
Assay range:5μmol/L -160μmol/L 48determinations
Purpose
This kit allows for the determination of LDL concentrations in Rat serum, cell culture简介:Rat Low density lipoprotein(LDL)
FOR RESEARCH USE ONLY
Assay range:5μmol/L -160μmol/L 48determinations
Purpose
This kit allows for the determination of LDL concentrations in Rat serum, cell culture supernates and other biological fluids
Principle of the assay
The kit assay Rat LDL level in the sample, use Purified Rat LDL antibody to coat microtiter plate wells, make solid-phase antibody, then add LDL to wells, Combined LDL antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat LDL in the samples is then determined by comparing the O.D. of the samples to the standard curve.详细>