简介:Rat CTx-I
FOR RESEARCH USE ONLY
Assay range:0.8nmol/L - 35nmol/L 96 determinations
Purpose
This kit allows for the determination of CTx-I concentrations in Rat serum, cell culture supernates and othe简介:Rat CTx-I
FOR RESEARCH USE ONLY
Assay range:0.8nmol/L - 35nmol/L 96 determinations
Purpose
This kit allows for the determination of CTx-I concentrations in Rat serum, cell culture supernates and other biological fluids
Principle of the assay
The kit assay Rat CTx-I level in the sample, use Purified Rat CTx-I antibody to coat microtiter plate wells, make solid-phase antibody, then add CTx-I to wells, Combined CTx-I antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat CTx-I in the samples is then determined by comparing the O.D. of the samples to the standard curve.详细>