简介:The kit assay Rat TRACP5b level in the sample, use Purified Rat TRACP5b antibody to coat microtiter plate wells, make solid-phase antibody, then add TRACP5b to wells, Combined TRACP5b antibody which With HRP labeled, bec简介:The kit assay Rat TRACP5b level in the sample, use Purified Rat TRACP5b antibody to coat microtiter plate wells, make solid-phase antibody, then add TRACP5b to wells, Combined TRACP5b antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat TRACP5b in the samples is then determined by comparing the O.D. of the samples to the standard curve.详细>