简介: Human TB-IgG
FOR RESEARCH USE ONLY
Drug Names
Generic Name:Human TB-IgG ELISA Kit.
Purpose
This kit allows for the determination of TB-IgG concentrations in Human serum, and ot简介: Human TB-IgG
FOR RESEARCH USE ONLY
Drug Names
Generic Name:Human TB-IgG ELISA Kit.
Purpose
This kit allows for the determination of TB-IgG concentrations in Human serum, and other biological fluids.
Principle of the assay
The kit assay TB-IgG level in the sample,use Purified antigen to coat microtiter plate wells, make solid-phase antibody, then add TB-IgG to wells, Combined With TB-IgG, after washing and removing non-combinative antibody and other components ,then Combined antigen which with HRP labeled become antigen – antibody - enzyme- antigen complex, after washing Completely, Add TMB substrate solution,, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. Compared with the CUTOFF value, according to this to judge TB-IgG exist in the sample or not.详细>