激活体系
    In both without and with the addition of the exogenous activation system S9-mix, the human sperm samples were exposed to cyclophosphamide at the concentrations in 20, 40, 60μg/ ml for 3.5 hours respectively before interspecific in vitro fertilization with zona-free hamster ova, then the karyotypes of human spermatozoa were analyzed.
    在未加和加入体外代谢激活体系S9混合物2种情况下,将人精子用最终浓度分别为20、40、60μg/ml环磷酰胺处理3.5h,然后与去透明带地鼠卵进行异种体外受精,继而制备精子染色体进行核型分析。