请教各位大神,我最近做液相色谱检测有机酸(酮戊二酸和苹果酸)检测不到吸收峰,只有基线,已排除样品问题。去年做的时候可以在12min左右出峰,现在流动相还是0.5%磷酸氢二铵缓冲液,波长214nm,流速0.5ml/min。请问是仪器故障吗?会不会出现紫外检测不到吸收峰的故障?谢谢! |
[img=,481,356]https://ng1.17img.cn/bbsfiles/images/2019/09/201909241048502998_9572_1847709_3.jpg!w481x356.jpg[/img][img=,690,213]https://ng1.17img.cn/bbsfiles/images/2019/09/201909241048505838_6451_1847709_3.jpg!w690x213.jpg[/img]