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共有 2 人回复了该问答哪些因素可以导致NOESY 中的cross peaks 变宽呢
 回复zyqfrog10发表于:2006/5/1 18:45:00悬赏金额:20积分 状态:未解决
有哪些因素可以导致NOESY 中的cross peaks 变宽(个别变宽)呢?
谢谢!

Welcome discussion!
 回复  1# zyqfrog10  回复于:2006/5/6 10:48:00
Actually it should be arised by the 1D peak broadening.
I checked some materials, and got three probabilities.

From Wuthrich's book:
  if the atom is kinetically stable,it should be related to intrisic T2 or pure T2;
  if the atom is active, there exists exchange processes,which cause line broadening;
  experimental artifacts, say the inhomogeneity of the static field Bo, which can be improved by shimming.

In protein NMR study, CPMG is usually employed to investigate the dynamics.
I recommend two articles:
Science,V.312,14 April,2006,Pp224-227;
JBNMR, V.31,No.4,April,2005,Pp337-342. 
They are inspiring.

(Sorry, there is no chinese system in our lab computer!)

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