5-羟色胺没有找到，但是有色胺的
LCMS检测方法可以参考，需要使用比较特殊的亲水柱来做。色胺比5-羟色胺更难保留，所以这个条件做5-羟色胺应该没有问题。
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| Column | Cogent Diamond Hydride™, 4µm, 100A |
| Catalog No. | 70000-15P-2 |
| Dimensions | 2.1 x 150 mm |
| Solvents | A: DI water + 0.1% ammonium formate pH 5.9
B: 90% acetonitrile/10% DI water w/ 0.1% ammonium formate pH 6 |
| Mobile Phase | Gradient t0 = 1.44 min
| Time | %B | Time | %B | | 0.0 | 90 | 7.0 | 70 | | 2.5 | 90 | 7.1 | 30 | | 6.0 | 70 | 8.0 | 30 |
|
| Flow Rate | 0.4 mL/min. |
| Sample | Mixture of 3 biogenic amines: - Tryptamine 159.09222 m/z (M-H)-, RT = 2.23 min
- Serotonin (5-hydroxytryptamine) 175.08714 m/z (M-H)-, RT = 2.63 min
- Dopamine (3-hydroxytyramine) 152.07 m/z (M-H)-, RT = 3.51 min
|
| Detection | ESI � neg - Agilent 6210 MSD TOF mass spectrometer. |
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