Cleaning: 20 volumes of the following in order: water, ACN, DCM, ACN, water for reversed phase. 20 volumes of the following for normal phase: Hexane, DCM, ACN, DCM, Hexane. For sugar the column should be washed with 0.1% diethylenetriamine or triethylenetetramine in 50/50 MeCN/water for about 100 column volumes and then used in the MeCN/water mobile phase necessary for getting good retention for his carbohydrates.