荧光素钠可以用HPLC-DAD/ESI-MS来测定。荧光素具有香豆素类化合物基本母核,在UV 280nm有较强的特征吸收,可以设为UV检测波长。 提供一个参考色谱条件: column:C18 UV: 280 nm Mobile phase consisted of (A)water-acetonitrile-acetic acid (89:9:2) and (B) water-acetonitrile(20:80). The separation was performed using a linear gradient from 0% to 30% B in 30 min. The structural information was obtained using a Finnigan LCQ ion trap mass spectrometer (Thermoquest, San Jose, CA) equipped with an API chamber and an ESI source. The ionization mode was negative mode;
参考文献:Development and Validation of an Improved Oxygen Radical Absorbance Capacity Assay Using Fluorescein as the Fluorescent Probe http://www.brunswicklabs.com/artman/uploads/jafc-boxin.pdf
The potential of nasal application for delivery to the central brain—a microdialysis study of fluorescein in rats