原文由 redanqi 发表:
G. SCREENING CRITERIA
1.
The retention time must match that of an external standard within 5%.
2.
The melamine product ion abundance ratios must match that of an external standard within 20% relative.
Example calculation:
Melamine ion ratio = (area of m/z 127 to 85 peak) ÷ (area of m/z 127 to 68 peak)
Note: Most mass spectrometers calculate the reciprocal of the above and state it as a percentage, which is equally valid.
Passing ion ratio of a sample = ion ratio of a standard ± (0.2 * ion ratio of a standard)
3.
The solvent blank, ACN:H2O (1:1, v/v), injected after the standard curve must be negative for melamine (no peak within the retention time window with passing ion ratio), demonstrating no carryover.
4.
The positive tissue control must be positive for melamine (meeting retention time and ion ratio criteria).
5.
The negative tissue control must not contain melamine at levels higher than 10% of the positive tissue control. Melamine peaks meeting the retention time and ion ratio criteria must be compared to the positive tissue control.
The Internal standard is used, the melamine quantification peak area (m/z 127 to 85) divided by the internal standard peak area (m/z 130 to 87) must be less than or equal to10% of that of the positive tissue control.
6. For a sample to be screen positive, it must be found to contain melamine at or above the 50ppb minimum proficiency level of this analytical method.
The internal standard is used, the melamine quantification peak area (m/z 127 to 85) divided by the internal standard peak area (m/z 130 to 87) must be greater than or equal to that of the positive tissue control.

原文由 redanqi 发表:
请先翻译附件中文件的A与G部分，最终评分按翻译质量和数量分配,同时欢迎各位版友参与评译，参与者亦有积分

A. INTRODUCTION
1.
Theory
Melamine is extracted from muscle tissue with a mixture of acetonitrile and water. The extract is cleaned up by liquid/liquid extraction with methylene chloride followed by solid phase extraction (SPE). Melamine is eluted from the SPE cartridge with 5% ammonium hydroxide / methanol, evaporated to dryness, reconstituted with internal standard and 50% acetonitrile / water, and analyzed by LC/MS/MS.
2.
Applicability
This method is applicable for screening of melamine in swine and poultry muscle at ≥ 50 ppb.
v

原文由 zhuxiugao 发表:

原文由 redanqi 发表: 请先翻译附件中文件的A与G部分，最终评分按翻译质量和数量分配,同时欢迎各位版友参与评译，参与者亦有积分 A. INTRODUCTION 1. Theory Melamine is extracted from muscle tissue with a mixture of acetonitrile and water. The extract is cleaned up by liquid/liquid extraction with methylene chloride followed by solid phase extraction (SPE). Melamine is eluted from the SPE cartridge with 5% ammonium hydroxide / methanol, evaporated to dryness, reconstituted with internal standard and 50% acetonitrile / water, and analyzed by LC/MS/MS. 2. Applicability This method is applicable for screening of melamine in swine and poultry muscle at ≥ 50 ppb. v

呵呵，俺先试试这一部分
介绍
1、 理论
三聚氰胺使用乙腈与水的混合物从肌肉组织中萃取，萃取物利用液液萃取方式通过SPE用二氯甲烷净化。三聚氰胺使用5%的氢氧化铵/甲醇从SPE柱中洗脱，蒸发至干燥，用内标物与50%的乙腈/水重新配置成原来的浓度，通过LC/MS/MS分析。
2、
适用范围
本方法适用于快速筛选分析猪与禽肌肉组织中三聚氰胺含量大于等于50ppb。