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LC–MS/MS is becoming the preferred method for quantitative determinations due to rapid, highly selective and sensitive analysis. LC–MS/MS methods using turbo-electrospray ionisation (ESI) for determination of levonorgestrel in human serum [5], environmental water [6] and sewage effluent [7] have been described, but the APPI source used in our laboratory lower the background noise produced by ESI, enabling us to develop a more sensitive method, with high sample throughput due to short chromatographic conditions and simple sample preparation. Kushnir et al. [9] used atmospheric pressure photo-ionisation with tandem mass spectrometry to analyse cortisol and cortisone in serum and plasma.
LC–MS/MS 方法因为其快速、高选择性和灵敏性，正逐渐成为定量分析的优选方法。利用具高速-电喷射离子化技术（turbo-electrospray ionisation (ESI)不懂）的 LC–MS/MS 系统测定人血清、环境水体和污水中左炔诺孕酮已有描述[7]，由于我们实验室使用的APPI源可以降低ESI产生的背景噪音，从而使我们能开发出一个更灵敏的方法。该方法因色谱运行时间短而能快速大量分析样品，制样也简单。Kushnir等[9]利用atmospheric pressure photo-ionisation with tandem mass spectrometry 分析了血清和血浆中的氢化可的松（cortisol）和可的松（ cortisone）的含量。

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